In the present study, we tested possible ways of modification of the context long-term memory using the reconsolidation as a tool. Recently, using a depletion of the serotonin content, it was shown that the reinforcing neurotransmitter serotonin is necessary for successful repeated reconsolidation of context memory in terrestrial snails Helix lucorum (Balaban et al. in Sci Rep 6:36933, 2016), and in the present study, we investigated effects of serotonin increase in memory maintenance by injection of the serotonin precursor 5-hydroxytryptophan (5-HTP). We studied reinstatement of the context memory after its impairment during reconsolidation with a protein synthesis blocker anisomycin (ANI) or with a specific inhibitor of protein-kinase Mζ (ZIP). It was observed that applications of 5-HTP alone, known to increase the release of serotonin, or reactivation of memory alone did not restore the ZIP- or ANI-impaired context memory, while combination of the 5-HTP + reactivation of memory effectively reinstated the context memory. The data obtained confirmed the assumption that serotonin/reinforcing transmitter is a part of successful reconsolidation necessary for memory maintenance, demonstrated possible ways of long-term memory regulation during the reconsolidation process.

在本研究中，我们测试了使用重新巩固作为工具来修改上下文长期记忆的可能方法。最近，通过消耗血清素含量，研究表明，增强神经递质血清素对于陆生蜗牛Helix lucorum成功重复重新巩固情境记忆是必要的（Balaban 等人，发表于 Sci Rep 6:36933, 2016），而在在本研究中，我们通过注射血清素前体 5-羟色氨酸 (5-HTP) 来研究血清素增加对记忆维持的影响。我们研究了在使用蛋白质合成阻滞剂茴香霉素（ANI）或特定的蛋白激酶Mz抑制剂（ZIP）进行再巩固过程中受损后情景记忆的恢复。据观察，单独使用已知可增加血清素释放的 5-HTP 或单独重新激活记忆并不能恢复 ZIP 或 ANI 受损的上下文记忆，而 5-HTP + 重新激活记忆的组合可以有效恢复上下文记忆。获得的数据证实了这样的假设，即血清素/强化递质是记忆维持所需的成功再巩固的一部分，证明了再巩固过程中长期记忆调节的可能方法。