RNA silencing describes a pan-eukaryotic pathway of gene regulation where doubled stranded RNA are processed by the RNAse III enzyme Dicer or homologs. In particular, plants use it as a way to defend themselves against pathogen invasions. In turn, to evade the plant immune response, viruses have developed anti-RNA silencing mechanisms. They may indeed code for proteins called “viral suppressor of RNA silencing” which block the degrading of viral genomic or messenger RNA by the plant. The Rice Mottle Virus is an African virus of the sobemovirus family, which attacks the most productive rice varieties cultivated on this continent. It encodes P1, a cysteine-rich protein described as a potential RNA silencing suppressor. P1 is a 157 amino-acid long protein, characterized by a high propensity to aggregate concomitant with a limited stability with time in the conditions used in structural studies. To overcome this problem, shorter fragments were also studied. This strategy enabled the assignment of more than 90% backbone resonances of P1. This assignment should set the base of future NMR investigation of the protein structure and of its interactions with rice cellular partners.

中文翻译：

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非洲稻黄斑驳病毒编码的病毒蛋白P1的NMR化学位移主链分配。

RNA沉默描述了基因调控的泛真核生物途径，其中双链RNA由RNAse III酶Dicer或同系物加工。特别是，植物将其用作防御病原体入侵的一种方法。反过来，为了逃避植物的免疫反应，病毒开发了抗RNA沉默机制。它们确实可以编码称为“ RNA沉默的病毒抑制剂”的蛋白质，该蛋白质可阻止植物降解病毒基因组或信使RNA。稻斑驳病毒是一种sobemovirus家族的非洲病毒，它攻击在该大陆上种植的生产力最高的水稻品种。它编码P1，一种被称为潜在的RNA沉默抑制剂的富含半胱氨酸的蛋白质。P1是157个氨基酸的长蛋白，在结构研究中所用的条件下，其特征是伴随聚集的倾向很高，并且随时间的推移具有有限的稳定性。为了克服这个问题，还研究了较短的片段。这种策略可以分配P1的90％以上的主干共振。该任务应为蛋白质结构及其与水稻细胞伴侣相互作用的未来NMR研究奠定基础。