Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the primary pathogens that causes severe economic losses to sericulture. Comparative transcriptomics analysis has been widely applied to explore the antiviral mechanism in resistant strains. Here, to identify genes involved in BmNPV infection, we identified differentially expressed genes (DEGs) and performed weighted gene co‐expression network analysis (WGCNA) between two Bombyx mori strains: strain 871 (susceptible to BmNPV infection) and the near‐isogenic strain 871C (resistant to BmNPV). Our results showed that 400 genes were associated with resistance in strain 871C, and 76 genes were related to susceptibility in strain 871. In addition, the correlation analysis of DEGs and WGCNA showed that 40 genes related to resistance were highly expressed in the resistant strain. Among them, gene BGIBMGA004291 was the most noticeable. We further identified the effect of gene BGIBMGA004291, which encoded a multiprotein bridge factor 2 (MBF2) family member (MBF2‐10), on viral infection in cells. Our data suggested that MBF2‐10 inhibited viral infection. Taken together, this study showed specific module trait correlations related to viral infection in strains 871 and 871C, and we identified a resistance‐related gene. These findings suggested promising candidate genes with antiviral activity, aiding in the analysis of the antiviral molecular mechanisms in resistant strains.

中文翻译：

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通过比较转录组分析，与家蚕易感和近等基因抗性菌株中的核多角体病毒相关的特定基因。

家蚕核多角体病毒（BmNPV）是导致蚕桑业严重经济损失的主要病原体之一。比较转录组学分析已广泛用于探索抗性菌株的抗病毒机制。在这里，为了鉴定涉及BmNPV感染的基因，我们鉴定了差异表达基因（DEG），并在两个家蚕之间进行了加权基因共表达网络分析（WGCNA）。菌株：871株（易受BmNPV感染）和近等基因株871C（抗BmNPV）。我们的结果表明，在871C菌株中有400个基因与抗性相关，在871菌株中76个基因与易感性相关。此外，DEG和WGCNA的相关分析表明，在抗性菌株中40个与抗性相关的基因高表达。其中，基因BGIBMGA004291最引人注意。我们进一步确定了基因BGIBMGA004291对细胞病毒感染的影响，该基因编码多蛋白桥因子2（MBF2）家族成员（MBF2-10）。我们的数据表明MBF2-10可抑制病毒感染。综上所述，这项研究显示了与871和871C株病毒感染相关的特定模块性状相关性，我们确定了一个与耐药性相关的基因。