ABSTRACT Igf2, H19, and Nctc1 are linked co-regulated genes on distal mouse chromosome 7. This locus is an important model both for studying mechanisms of monoallelic expression and for elucidating the role of cis-regulatory elements – enhancers and insulators – in organizing chromatin and gene expression across a large domain. In this study we characterize regulated expression of the Igf2 antisense transcript (Igf2as) in primary muscle cells. We demonstrate that Igf2as is imprinted (expressed only from the paternal chromosome). We also show that Igf2as expression during differentiation follows the same patterns as Igf2 and H19. Moreover, this expression is dependent upon the same shared enhancer element. Thus, our work shows that the imprinted cluster includes Igf2as in addition to H19, Igf2, and Nctc1.

中文翻译：

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Igf2as 的父本偏向性表达依赖于肌细胞中 Igf2、H19 和 Nctc1 的印记簇

摘要 Igf2、H19 和 Nctc1 是小鼠远端 7 号染色体上连锁的共调控基因。该基因座是研究单等位基因表达机制和阐明顺式调控元件（增强子和绝缘子）在组织染色质中的作用的重要模型和跨大域的基因表达。在这项研究中，我们表征了原代肌肉细胞中 Igf2 反义转录物 (Igf2as) 的受调控表达。我们证明 Igf2as 是有印记的（仅从父本染色体表达）。我们还表明分化过程中 Igf2as 的表达遵循与 Igf2 和 H19 相同的模式。此外，该表达依赖于相同的共享增强子元件。因此，我们的工作表明，除了 H19、Igf2 和 Nctc1 之外，印记集群还包括 Igf2as。