ABSTRACT Hereditary retinal disease (HRD) is the primary retinal degeneration that leads to severe visual impairments and refractory blindness, and the therapy of HRD was most important in ophthalmology. The apoptosis of retinal cells plays important roles in HRD progression. Therefore, in this study, we explore the mechanism of H2O2 and blue light-induced apoptosis of ARPE-19 cells. Co-immunoprecipitation (Co-IP) is employed to test the interactions between proteins, and western blotting is used to detect the protein levels. Apoptosis is analyzed by Flow cytometry. Our results found that PRDX6 could interact with RARA in ARPE-19 cells, and H2O2 and blue light could significantly reduce the RARA protein expression, and also could inhibit the interaction between PRDX6 and RARA. Using a rescue experiment, we further elucidated that H2O2 and blue light reduced the RARA expression via down-regulating PRDX6. And H2O2 and blue light induced the ARPE-19 cell apoptosis via decreasing the expression of PRDX6. Our results suggested that the interaction between PRDX6 and RARA played important roles in the apoptosis of ARPE-19 cells.

中文翻译：

---

PRDX6通过下调和与RARA相互作用调节H2O2和蓝光诱导的APRE-19细胞凋亡

摘要 遗传性视网膜疾病（HRD）是导致严重视力障碍和难治性失明的原发性视网膜变性，HRD的治疗在眼科中最为重要。视网膜细胞的凋亡在 HRD 进展中起重要作用。因此，在本研究中，我们探讨了 H2O2 和蓝光诱导 ARPE-19 细胞凋亡的机制。免疫共沉淀 (Co-IP) 用于测试蛋白质之间的相互作用，蛋白质印迹用于检测蛋白质水平。通过流式细胞术分析细胞凋亡。我们的结果发现PRDX6可以与ARPE-19细胞中的RARA相互作用，H2O2和蓝光可以显着降低RARA蛋白的表达，也可以抑制PRDX6与RARA之间的相互作用。使用救援实验，我们进一步阐明 H2O2 和蓝光通过下调 PRDX6 降低了 RARA 的表达。H2O2和蓝光通过降低PRDX6的表达诱导ARPE-19细胞凋亡。我们的结果表明 PRDX6 和 RARA 之间的相互作用在 ARPE-19 细胞的凋亡中起重要作用。