Background: Oral biofilms are the root cause of major oral diseases. As in vitro biofilms are not representative of the intraoral milieu, various devices have been manufactured over the years to develop Appliance Grown Oral Biofilm (AGOB).

Objective: To review various intraoral appliances used to develop AGOB for microbiological analysis, and to judge the optimal means for such analyses.

Design: Four databases (PubMed, Science Direct, Scopus and Medline) were searched by two independent reviewers, and articles featuring the key words ‘device’ OR ‘splint’ OR ‘appliance’; ‘Oral biofilm’ OR ‘dental plaque’; ‘in vivo’ OR ‘in situ’; ‘Microbiology’ OR ‘Bacteria’ OR ‘microbiome’; were included. The standard Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) were adopted for data gathering.

Results: Of the 517 articles which met the initial inclusion criteria, 24 were deemed eligible for review. The age of the AGOB, sampled at various intervals, ranged from 30 min to 28 days. The most commonly used microbiome analytical methods were fluorescence microscopy, total cell count using conventional, and molecular tools including Next Generation Sequencing (NGS) platforms.

Conclusions: No uniformly superior method for collecting AGOB could be discerned. NGS platforms are preferable for AGOB analyses.

背景：口腔生物膜是主要口腔疾病的根本原因。由于体外生物膜不能代表口腔内环境，因此多年来制造了各种设备来开发矫正器生长型口腔生物膜（AGOB）。

目的：综述用于开发AGOB进行微生物分析的各种口腔内器具，并判断进行此类分析的最佳方法。

设计：两名独立的审阅者搜索了四个数据库（PubMed，Science Direct，Scopus和Medline），并撰写了以关键词“ device”或“ splint”或“ appliance”为关键词的文章；“口腔生物膜”或“牙菌斑”；“体内”或“原位”；“微生物学”或“细菌”或“微生物组”；被包括在内。采用标准的系统评价和荟萃分析报告项目（PRISMA）进行数据收集。

结果：在符合最初纳入标准的517篇文章中，有24篇被认为符合审查条件。以不同的时间间隔采样的AGOB的年龄为30分钟至28天。最常用的微生物组分析方法是荧光显微镜检查，使用常规方法的总细胞计数以及包括下一代测序（NGS）平台在内的分子工具。

结论：尚无统一的优良方法收集AGOB。对于AGOB分析，NGS平台更可取。