Binge drinking is defined as the infrequent consumption of excessive doses of alcohol in a short period of time. Zebrafish is a reliable model to investigate ethanol consumption impact on the CNS, including reward signaling like dopaminergic neurotransmission system. The aim of this study was to evaluate zebrafish brain dopaminergic parameters after intermittent weekly ethanol exposure (WEE), which mimics binge drinking. Thus, adult zebrafish were exposed to ethanol (1.4% v/v) for 30 min, once a week for three consecutive weeks. The groups were divided according to different time points after the third exposure and name as follow: immediately (WEEI), two days (WEE-2), and nine days (WEE-9) after last exposure to ethanol. Brain dopaminergic function was assessed by the activity of the dopamine transporters (DAT); monoamine oxidase (MAO) activity; dopamine and noradrenaline levels by chromatography. The WEE-I and WEE-2 groups presented a significant increase in DAT activity. The MAO activity was decreased for WEE-2 and WEE-9 groups. The WEE-2 and WEE-9 groups presented an increase in brain dopamine levels, while noradrenaline levels were not affected. Therefore, dopaminergic parameters are still altered two and nine days after the last ethanol exposure in this binge experimental model, resulting in a modulatory event in this neurotransmission pathway.

中文翻译：

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每周乙醇暴露会改变斑马鱼大脑中的多巴胺能参数。

暴饮暴食的定义是在短时间内不经常食用过量的酒精。斑马鱼是研究乙醇消耗对中枢神经系统影响的可靠模型，包括像多巴胺能神经传递系统这样的奖励信号。这项研究的目的是评估间歇性每周乙醇暴露（WEE）后的斑马鱼脑多巴胺能参数，该模拟模仿暴饮暴食。因此，成年斑马鱼每周一次连续三周暴露于乙醇（1.4％v / v）中30分钟。第三次接触后，根据不同的时间点将各组划分为以下名称：最后一次接触乙醇后立即（WEEI），两天（WEE-2）和九天（WEE-9）。通过多巴胺转运蛋白（DAT）的活性评估脑多巴胺能功能；单胺氧化酶（MAO）活性；通过色谱分析多巴胺和去甲肾上腺素水平。WEE-1和WEE-2组的DAT活性显着增加。WEE-2和WEE-9组的MAO活性降低。WEE-2和WEE-9组的脑多巴胺水平升高，而去甲肾上腺素水平未受影响。因此，在这种暴饮暴食的实验模型中，最后一次乙醇暴露后的第二天和第九天，多巴胺能参数仍然会发生变化，从而导致该神经传递途径发生调节事件。