Human myeloid angiogenic cells (MACs), also termed early endothelial progenitor cells, play an important role in neovascularization and vascular repair. MicroRNAs (miRNAs) are a class of naturally occurring, noncoding, short (∼22 nucleotides), single-stranded RNAs that regulate gene expression post-transcriptionally. MiRNAs have been shown to regulate MAC function. A miRNA signature of MACs was described approximately a decade ago, and many new miRNAs have been discovered in recent years. In this study, we aimed to provide an up-to-date miRNA signature for human MACs. MACs were obtained by culture of human peripheral blood mononuclear cells in endothelial medium for 7 days. Using qPCR array analysis we identified 72 highly expressed miRNAs (CT value < 30) in human MACs. RT-qPCR quantification of select miRNAs revealed a strong correlation between the CT values detected by the array analysis and RT-qPCR, suggesting the miRNA signature generated by the qPCR array assay is accurate and reliable. Experimentally validated target genes of the 10 most highly expressed miRNAs were retrieved. Only a few of the targets and their respective miRNAs have been studied for their role in MAC biology. Our study therefore provides a valuable repository of miRNAs for future exploration of miRNA function in MACs.

中文翻译：

---

从外周血单核细胞衍生的人髓样血管生成细胞的MicroRNA分析。

人骨髓血管生成细胞（MAC），也称为早期内皮祖细胞，在新血管形成和血管修复中起重要作用。微小RNA（miRNA）是一类天然存在的非编码短（〜22个核苷酸）单链RNA，可在转录后调节基因表达。MiRNA已显示出调节MAC功能的作用。MAC的miRNA签名描述于大约十年前，并且近年来发现了许多新的miRNA。在这项研究中，我们旨在为人类MAC提供最新的miRNA签名。通过在内皮培养基中培养人外周血单个核细胞7天获得MAC。使用qPCR阵列分析，我们在人的MAC中鉴定出72个高度表达的miRNA（CT值<30）。选定miRNA的RT-qPCR定量显示，通过阵列分析检测到的CT值与RT-qPCR之间存在很强的相关性，这表明qPCR阵列测定产生的miRNA签名是准确可靠的。检索了10个表达最高的miRNA的经实验验证的靶基因。仅研究了一些靶标及其各自的miRNA在MAC生物学中的作用。因此，我们的研究为将来探索MAC中的miRNA功能提供了有价值的miRNA储存库。仅研究了一些靶标及其各自的miRNA在MAC生物学中的作用。因此，我们的研究为将来探索MAC中的miRNA功能提供了有价值的miRNA储存库。仅研究了一些靶标及其各自的miRNA在MAC生物学中的作用。因此，我们的研究为将来探索MAC中的miRNA功能提供了有价值的miRNA储存库。