Cytoskeletal actin dynamics are crucial for the activation of T-cells. Immortalised Jurkat T-cells have been the model system of choice to examine and correlate the dynamics of the actin cytoskeleton and the immunological synapse leading to T-cell activation. However, it has remained unclear whether immortalised cellular systems, such as Jurkat T-cells can recapitulate the cytoskeletal behaviour of primary T-cells. Studies delineating the cytoskeletal behaviour of Jurkat T-cells in comparison to primary T-cells are lacking. Here, we employ live-cell super-resolution microscopy to investigate the cytoskeletal actin organisation and dynamics of living primary and immortalised Jurkat T-cells at the appropriate spatiotemporal resolution. Under comparable activation conditions, we found differences in the architectural organisation and dynamics of Jurkat and primary mouse and human T-cells. Although the three main actin network architectures in Jurkat T-cells were reminiscent of primary T-cells, there were differences in the organisation and molecular mechanisms underlying these networks. Our results highlight mechanistic distinctions in the T-cell model system most utilised to study cytoskeletal actin dynamics.

中文翻译：

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原发性和永生化T细胞中不同的肌动蛋白细胞骨架行为。

细胞骨架肌动蛋白动力学对于T细胞的激活至关重要。永生化的Jurkat T细胞已成为检查和关联肌动蛋白细胞骨架的动态变化和导致T细胞活化的免疫突触的首选模型系统。然而，尚不清楚永生化的细胞系统（如Jurkat T细胞）能否概括初级T细胞的细胞骨架行为。缺乏描述Jurkat T细胞与原代T细胞相比的细胞骨架行为的研究。在这里，我们采用活细胞超高分辨率显微镜，以适当的时空分辨率研究活的原代和永生化Jurkat T细胞的细胞骨架肌动蛋白组织和动力学。在可比的激活条件下，我们发现Jurkat以及原代小鼠和人类T细胞在结构组织和动力学方面存在差异。尽管Jurkat T细胞中的三种主要肌动蛋白网络结构让人想起原代T细胞，但这些网络的组织和分子机制却存在差异。我们的研究结果突显了T细胞模型系统中最常用于研究细胞骨架肌动蛋白动力学的机制差异。