Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a rapid, cost-effective method for identification of a broad range of bacterial taxa, but its accuracy for Vibrio spp. from samples of aquatic animal origin is unknown. We used DNA sequence analysis targeting two conserved genes, rpoB and rpoD, as the identification standard for 5 reference strains and 35 Vibrio spp. field isolates obtained from diagnostic aquaculture samples. Overall, MALDI-TOF MS correctly identified 100% of the five reference strains to the genus level and 80% (4 of 5) to the species level. For field isolates, 83% (29 of 35) were correctly identified to the genus level, and 49% (17 of 35) were correctly identified to the species level. Eight (23%) field isolates were incorrectly identified at the species level. The MALDI-TOF MS method produced no identification for 17% (6 of 35) of the field isolates. Using traditional culture identification, 100% of the five reference strains were correctly identified to the species level. All 35 field isolates were correctly identified to the genus level; 51% (18 of 35) of the isolates were identified correctly to the species level, while 29% (10 of 35) were misidentified at the species level. Overall, MALDI-TOF MS was comparable to phenotypic identification, and accuracy will likely improve with enhancement of MALDI-TOF MS database robustness.

中文翻译：

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基质辅助激光解吸电离飞行时间质谱法在鱼类致病性弧菌鉴定中的应用

基质辅助激光解吸电离飞行时间质谱 (MALDI-TOF MS) 是一种快速、经济高效的方法，可用于识别广泛的细菌分类群，但其对弧菌属的准确性不高。来自水生动物来源的样本是未知的。我们使用针对两个保守基因 rpoB 和 rpoD 的 DNA 序列分析作为 5 种参考菌株和 35 种弧菌的鉴定标准。从诊断性水产养殖样品中获得的现场分离物。总体而言，MALDI-TOF MS 在属水平上 100% 正确识别了五个参考菌株，在种水平上正确识别了 80%（5 个中的 4 个）。对于野外分离株，83%（35 个中的 29 个）被正确鉴定为属水平，49%（35 个中的 17 个）被正确鉴定为物种水平。在物种水平上错误地识别了八个 (23%) 野外分离物。MALDI-TOF MS 方法对 17%（35 个中的 6 个）的现场分离株没有进行鉴定。使用传统的培养鉴定，5 个参考菌株 100% 被正确鉴定到物种水平。所有 35 个野外分离株均被正确鉴定为属水平；51%（35 个中的 18 个）在物种水平上被正确识别，而 29%（35 个中的 10 个）在物种水平上被错误识别。总体而言，MALDI-TOF MS 与表型鉴定相当，随着 MALDI-TOF MS 数据库稳健性的增强，准确性可能会提高。51%（35 个中的 18 个）分离株在物种水平上被正确识别，而 29%（35 个中的 10 个）在物种水平上被错误识别。总体而言，MALDI-TOF MS 与表型鉴定相当，随着 MALDI-TOF MS 数据库稳健性的增强，准确性可能会提高。51%（35 个中的 18 个）分离株在物种水平上被正确识别，而 29%（35 个中的 10 个）在物种水平上被错误识别。总体而言，MALDI-TOF MS 与表型鉴定相当，随着 MALDI-TOF MS 数据库稳健性的增强，准确性可能会提高。