Reference genes are critical to obtain reliable results of reverse transcription real-time quantitative PCR (RT-qPCR), which is widely used for relative quantification of gene expression. In this study, we evaluated the validity of seven candidate reference genes for normalization in RT-qPCR analysis in the deep-sea bacterium Shewanella psychrophila WP2 under different environmental conditions. Among the set of genes investigated, gyrA, 16S rRNA and rho were identified as the most suitable reference genes for WP2 at different temperatures, hydrostatic pressures and salinities, respectively. Notably, the rho gene is conserved in Shewanella genus and other deep-sea bacteria, thus, could be used as a versatile reference gene for RT-qPCR analysis of these microorganisms under extreme environmental conditions.

中文翻译：

---

验证参考基因用于深海细菌嗜酸乳杆菌WP2中的逆转录实时定量PCR分析。

参考基因对于获得可靠的逆转录实时定量PCR（RT-qPCR）结果至关重要，RT-qPCR被广泛用于基因表达的相对定量。在这项研究中，我们评估了7种候选参考基因在不同环境条件下在深海细菌希瓦氏菌WP2中进行RT-qPCR分析标准化的有效性。在所研究的基因组中，分别在不同的温度，静水压力和盐度下，gyrA，16S rRNA和rho被确定为WP2最合适的参考基因。值得注意的是，rho基因在希瓦氏菌属和其他深海细菌中是保守的，因此可用作极端环境条件下这些微生物的RT-qPCR分析的通用参考基因​​。