Leifsonia sp. ku-ls is an endophytic bacterial strain colonizing in high numbers the stem and leaf of the high-yielding and widely grown indica rice cultivar RP Bio-226. Whole genome sequencing of this strain using Illumina Hiseq-2500 system resulted in generation of 10,103,994 paired-end reads of 150 nucleotides length. De novo assembly of the reads with A5MySeq resulted in 51 scaffolds. Kmer analysis with KAT estimated the genome size as 3.83 Mbp with 70% GC content. Annotation of the genome resulted in identification of 3930 protein-coding genes, 45 tRNA genes, and 3 rRNA genes. Detailed analysis of the genes predicted resulted in identification of host beneficial genes which include genes associated with hormone production, nitrogen metabolism, and stress response. There is an elaborate defense against oxidative stress present in this bacterium which also can mitigate plant oxidative stress resulting from disease/abiotic stress. Comparison of this endophytic bacterial genome with non-endophytic Leifsonia sp. showed presence of additional genes, increase in copy number of some of the genes and regulators. Many genes with eukaryotic-like domains have also been identified in this bacterium.

中文翻译：

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水稻内生Leifsonia sp。的全基因组测序和宿主相互作用基因的鉴定。ku-ls。

莱福索尼亚sp。ku-ls是一种内生细菌菌株，在高产量且广泛种植的in稻品种RP Bio-226的茎和叶上大量繁殖。使用Illumina Hiseq-2500系统对该菌株进行全基因组测序，结果产生了10,103,994个150核苷酸长的配对末端读数。从头开始使用A5MySeq进行组装，结果产生了51个支架。用KAT进行的Kmer分析估计基因组大小为3.83 Mbp，GC含量为70％。对基因组的注释导致鉴定了3930个蛋白质编码基因，45个tRNA基因和3个rRNA基因。对预测基因的详细分析导致鉴定出宿主有益基因，其中包括与激素产生，氮代谢和应激反应相关的基因。在这种细菌中存在针对氧化应激的精心防御，这也可以减轻由疾病/非生物胁迫导致的植物氧化应激。该内生细菌基因组与非内生细菌的比较利福尼亚菌 显示存在其他基因，某些基因和调节子的拷贝数增加。在该细菌中还鉴定出许多具有真核样结构域的基因。