OBJECTIVES β-lactoglobulin (β-Lg)-sensitized mice model was employed to investigate the correlation between Lactobacillus acidophilus KLDS 1.0738 (Lap KLDS 1.0738) modulating gut microbiota and inducting Toll-like receptors (TLRs) expression. METHODS The alterations of mice fecal microbiota were analyzed by 16S rRNA gene sequencing. The serum cytokines production and TLR4/NF-κB mRNA expression in the colon tissues were measured by ELISA kit and quantitative RT-PCR, respectively. RESULTS The results showed that Lap KLDS 1.0738 pretreatment attenuated β-Lg-induced hypersensitivity, accompanied with a diminished expression of TLR4/NF-κB signaling. Moreover, oral administration of Lap KLDS 1.0738 improved the richness and diversity of fecal microbiota, which was characterized by fewer Proteobacteria phylum and Helicobacteraceae family, and higher Firmicutes phylum and Lachnospiraceae family than allergic group. Notably, TLR4/NF-κB expression was positively correlated with the family of Helicobacteraceae in allergic group, but negatively correlated with the family of Lachnospiraceae, Ruminococcaceae and anti-inflammatory cytokines level. A significant positive correlation was observed between TLR4/NF-κB expression and the production of histamine, total IgE and pro-inflammatory cytokines. CONCLUSIONS Intake of Lap KLDS 1.0738 can influence the gut bacterial composition, which might result in recognizing TLRs signaling so as to inhibit allergic response.

中文翻译：

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嗜酸乳杆菌KLDS 1.0738对β-乳球蛋白诱导的过敏小鼠模型中肠道菌群和TLR4表达的调节作用。

目的使用β-乳球蛋白（β-Lg）致敏的小鼠模型研究嗜酸乳杆菌KLDS 1.0738（Lap KLDS 1.0738）调节肠道菌群与诱导Toll样受体（TLRs）表达之间的相关性。方法采用16S rRNA基因序列分析小鼠粪便菌群的变化。用ELISA试剂盒和定量RT-PCR分别检测结肠组织中血清细胞因子的产生和TLR4 /NF-κBmRNA的表达。结果结果表明，Lap KLDS 1.0738预处理减弱了β-Lg诱导的超敏反应，并伴有TLR4 /NF-κB信号表达的减少。此外，Lap KLDS 1.0738口服可改善粪便微生物群的丰富性和多样性，其特点是门氏杆菌和幽门螺杆菌家族较少，并且比过敏组高的硬毛门和鞭毛科。值得注意的是，在变态反应组中，TLR4 /NF-κB表达与幽门螺杆菌家族呈正相关，而与鼻螺旋藻科，瘤胃球菌科和抗炎细胞因子水平呈负相关。TLR4 /NF-κB表达与组胺，总IgE和促炎细胞因子的产生之间存在显着正相关。结论膝部KLDS 1.0738的摄入可影响肠道细菌组成，这可能导致识别TLRs信号传导从而抑制变态反应。但与漆螺科，瘤胃科和消炎细胞因子水平呈负相关。在TLR4 /NF-κB表达与组胺，总IgE和促炎细胞因子的产生之间存在显着的正相关。结论膝部KLDS 1.0738的摄入可影响肠道细菌组成，这可能导致识别TLRs信号传导从而抑制变态反应。但与漆螺科，瘤胃科和消炎细胞因子水平呈负相关。TLR4 /NF-κB表达与组胺，总IgE和促炎细胞因子的产生之间存在显着正相关。结论膝部KLDS 1.0738的摄入可影响肠道细菌的组成，这可能导致识别TLRs信号传导，从而抑制变态反应。