The biologic function as well as the mechanism of long noncoding RNAs (lncRNAs) in colorectal cancer (CRC) still remain largely unknown. Long noncoding RNA FGD5 antisense RNA 1 (FGD5-AS1) has been reported to have a promotive effect on other human cancers, but its function in CRC still remains unknown. The expression levels of long noncoding RNA FGD5-AS1, CDCA7 mRNA, and miR-302e were assessed by RT-qPCR. The protein levels of CDCA7 were assessed by Western blot. The function of FGD5-AS1 was detected using cell viability assay, 5-ethynyl-2'-deoxyuridine (EdU) assay, transwell, and caspase-3 activity assay. Additionally, the microRNAs (miRNAs) sponge potential of FGD5-AS1 was examined by RNA immunoprecipitation assay, RNA pull-down assay, and luciferase reporter assay. FGD5-AS1 was increased in colorectal cancer cell lines compared to normal cell lines. Inhibition of FGD5-AS1 suppressed cell proliferation, migration, invasion, and accelerated cell apoptosis in CRC. FGD5-AS1 competitively bound with miR-302e to modulate CDCA7. The inhibiting effects of FGD5-AS1 knockdown on CRC cell proliferation, migration, and invasion, and the promoting effects on CRC cell apoptosis could be revived by miR-302e suppression or CDCA7 upregulation. LncRNA FGD5-AS1 could promote CRC progression through sponging miR-302e and upregulating CDCA7. FGD5-AS1 might serve as a potential therapeutic target for CRC.

中文翻译：

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长的非编码RNA FGD5-AS1通过海绵miR-302e上调CDCA7促进结直肠癌细胞的增殖，迁移和侵袭。

结直肠癌（CRC）的生物学功能以及长非编码RNA（lncRNA）的机制仍然未知。据报道，长的非编码RNA FGD5反义RNA 1（FGD5-AS1）对其他人类癌症具有促进作用，但其在CRC中的功能仍然未知。通过RT-qPCR评估长非编码RNA FGD5-AS1，CDCA7 mRNA和miR-302e的表达水平。通过蛋白质印迹评估CDCA7的蛋白质水平。使用细胞活力测定，5-乙炔基-2'-脱氧尿苷（EdU）测定，transwell和caspase-3活性测定来检测FGD5-AS1的功能。另外，通过RNA免疫沉淀测定，RNA下拉测定和荧光素酶报告基因测定来检查FGD5-AS1的微小RNA（miRNA）海绵潜力。与正常细胞系相比，FGD5-AS1在大肠癌细胞系中增加。抑制FGD5-AS1可抑制CRC中的细胞增殖，迁移，侵袭和加速细胞凋亡。FGD5-AS1与miR-302e竞争性结合以调节CDCA7。可以通过miR-302e抑制或CDCA7上调恢复FGD5-AS1敲低对CRC细胞增殖，迁移和侵袭的抑制作用，以及对CRC细胞凋亡的促进作用。LncRNA FGD5-AS1可以通过使miR-302e海绵化和上调CDCA7来促进CRC进展。FGD5-AS1可能作为CRC的潜在治疗靶标。可以通过miR-302e抑制或CDCA7上调恢复FGD5-AS1敲低对CRC细胞增殖，迁移和侵袭的抑制作用，以及对CRC细胞凋亡的促进作用。LncRNA FGD5-AS1可以通过使miR-302e海绵化和上调CDCA7来促进CRC进展。FGD5-AS1可能作为CRC的潜在治疗靶标。可以通过miR-302e抑制或CDCA7上调恢复FGD5-AS1敲低对CRC细胞增殖，迁移和侵袭的抑制作用，以及对CRC细胞凋亡的促进作用。LncRNA FGD5-AS1可以通过使miR-302e海绵化和上调CDCA7来促进CRC进展。FGD5-AS1可能作为CRC的潜在治疗靶标。