Presented here are two engineered bacterial biosensors for detecting and quantifying silver and copper ions. The biosensors contain a silver/copper resistance operon and a Green Fluorescent Protein gene that is strictly regulated through silver activated promoter regions normally found on a silver resistance gene (sil operon). The two biosensors efficiently detected silver and copper concentrations of 40 µM-300 µM and 20 µM-600 µM respectively. A strong correlation (R2 = 0.90 or above) between silver/copper and GFP signal makes it possible to quantify the ions using a linear regression. At room temperature incubation, the GFP signal of the biosensors in Ag+ saturated after 13 h. However, a detectable GFP signal was seen in 4 h.

中文翻译：

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经过设计的GFP荧光细菌生物传感器，用于检测和定量银和铜离子。

这里介绍了两个工程细菌生物传感器，用于检测和定量银和铜离子。生物传感器包含一个抗银/铜操纵子和一个绿色荧光蛋白基因，该基因通过通常在抗银基因（操纵子）上发现的银激活启动子区域受到严格调节。这两个生物传感器分别有效检测出40 µM-300 µM和20 µM-600 µM的银和铜浓度。银/铜和GFP信号之间的强相关性（R2 = 0.90或更高）使得可以使用线性回归对离子进行定量。在室温下孵育后，Ag +中生物传感器的GFP信号在13 h后达到饱和。但是，在4小时内发现了可检测到的GFP信号。