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Application of kDNA Minicircle PCR-RFLP to Characterize Leishmania donovani Clinical Isolates Obtained from Post-Kala-Azar Dermal Leishmaniasis in Eastern Nepal.
Canadian Journal of Infectious Diseases and Medical Microbiology ( IF 2.6 ) Pub Date : 2019-07-30 , DOI: 10.1155/2019/9392414
Ojesh Pokhrel 1 , Keshav Rai 1 , Narayan Raj Bhattarai 1 , Suman Rijal 2 , Arpana Rijal 3 , Basudha Khanal 1
Affiliation  

Post-kala-azar dermal leishmaniasis (PKDL) is a skin manifestation of visceral leishmaniasis (VL) which develops after apparent cure in some patients. PKDL is considered as the potential reservoir for the VL infection. Molecular epidemiological characterization of L. donovani isolates obtained from VL and PKDL isolates is essentially required in order to understand the transmission dynamics of the VL infection. To date, genetic variation among the VL and PKDL L. donovani isolates was not fully elucidated. Therefore, 14 clinical isolates from VL and 4 clinical isolates from PKDL were speciated by hsp70 and rDNA genes. Further characterization of L. donovani by haspB PCR demonstrates two different genotypes. All PKDL isolates have the same genetic structure. kDNA PCR-RFLP assay revealed 18 different genotypes; however, structural analysis showed the two distinct kDNA genotype population (k = 2). The kDNA fingerprint patterns of parasites from hilly districts were clustered separately from low-land districts. Therefore, further study with a large number of samples is urgently required for systematic characterization of the clinical isolates to track the molecular epidemiology of the Leishmania donovani causing VL and the role of PKDL as a reservoir.

中文翻译:

kDNA微型圆圈PCR-RFLP在表征尼泊尔东部卡拉-阿扎尔人皮肤利什曼病后获得的利什曼原虫临床分离株中的应用。

黑热病后皮肤利什曼病(PKDL)是内脏利什曼病(VL)的一种皮肤表现,在某些患者经明显治愈后会发展。PKDL被认为是VL感染的潜在库。的分子流行病学表征杜氏利什曼原虫分离株获得的VL和PKDL隔离以便了解VL感染的传播动力学基本上必需的。迄今为止,尚未完全阐明VL和PKDL donovani分离株之间的遗传变异。因此,由hsp70和rDNA基因确定了VL的14种临床分离株和PKDL的4种临床分离株。haspBL. donovani的进一步表征PCR显示出两种不同的基因型。所有PKDL分离物均具有相同的遗传结构。kDNA PCR-RFLP分析揭示了18种不同的基因型。但是,结构分析显示了两个不同的kDNA基因型种群(k  = 2)。丘陵地区寄生虫的kDNA指纹图谱与低地地区分开聚类。因此,迫切需要对大量样品进行进一步研究,以对临床分离株进行系统表征,以追踪引起VL的Leishmania donovani分子流行病学以及PKDL作为储库的作用。
更新日期:2019-07-30
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