Tissue engineering is a multidisciplinary field of research in which the cells, biomaterials, and processes can be optimized to develop a tissue substitute. Three-dimensional (3D) architectural features from electrospun scaffolds, such as porosity, tortuosity, fiber diameter, pore size, and interconnectivity have a great impact on cell behavior. Regarding tissue development in vitro, culture conditions such as pH, osmolality, temperature, nutrient, and metabolite concentrations dictate cell viability inside the constructs. The effect of different electrospun scaffold properties, bioreactor designs, mesenchymal stem cell culture parameters, and seeding techniques on cell behavior can be studied individually or combined with phenomenological modeling techniques. This work reviews the main culture and scaffold factors that affect tissue development in vitro regarding the culture of cells inside 3D matrices. The mathematical modeling of the relationship between these factors and cell behavior inside 3D constructs has also been critically reviewed, focusing on mesenchymal stem cell culture in electrospun scaffolds.

中文翻译：

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电纺支架中的间充质干细胞培养：组织工程的机械模型


组织工程是一个多学科研究领域，可以优化细胞、生物材料和工艺来开发组织替代品。电纺支架的三维 (3D) 结构特征，如孔隙率、弯曲度、纤维直径、孔径和互连性对细胞行为有很大影响。关于体外组织发育，pH、渗透压、温度、营养物和代谢物浓度等培养条件决定了构建体内的细胞活力。不同的电纺支架特性、生物反应器设计、间充质干细胞培养参数和播种技术对细胞行为的影响可以单独研究或与现象学建模技术相结合。这项工作回顾了影响 3D 基质内细胞培养的体外组织发育的主要培养和支架因素。这些因素与 3D 结构内细胞行为之间关系的数学模型也受到了严格的审查，重点关注电纺支架中的间充质干细胞培养。