Abstract

The functioning of NAD(P)H:FMN‑oxidoreductase (Red) from Vibrio fischeri under conditions of macromolecular crowding (MMC) simulated in vitro by adding biopolymers (starch and gelatin) was studied. The dissociation rate constants and the activation energies of dissociation of Red to the subunits were calculated, and the process of denaturation of Red was analyzed. It is shown that the functioning of Red both under conditions of MMC and in diluted solutions is the same. This result refutes the common belief that the native conformation of enzymes in vivo is stabilized due to MMC as compared to the in vitro conditions.

中文翻译：

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NAD（P）H：FMN-氧还原酶在大分子拥挤下的功能：体外建模。

摘要

研究了通过添加生物聚合物（淀粉和明胶）在体外模拟的大分子拥挤（MMC）条件下费氏弧菌的NAD（P）H：FMN-氧化还原酶（红色）的功能。计算了Red的离解速率常数和解离的活化能，并对Red的变性过程进行了分析。结果表明，Red在MMC条件下和稀释溶液中的功能均相同。该结果驳斥了这样一种普遍的信念，即与体外条件相比，MMC可稳定体内酶的天然构象。