BACKGROUND The role of dengue virus in altering the functional properties of platelets remains poorly understood. Few studies have observed that changes in fatty acids are found to have an effect on platelet activation and aggregation. Also, platelet fatty acids have not been extensively studied in dengue so far. So, we aimed to study the fatty acids of platelet membranes in patients with dengue. METHODS Gas chromatography-mass spectrometry (GC-MS) method was used to analyze fatty acids in the lipid extracts of platelets isolated from the study participants. RESULTS GC-MS analysis of platelet lipids identified and quantified nearly 23 unique lipid molecules on platelet membrane. We observed significant alterations with some of the fatty acids in patients with dengue compared to controls. Within dengue cases, increase in unsaturated fatty acids in severe dengue was observed compared to non-severe dengue. From baseline to defervescence, no difference in fatty acids was observed in dengue platelets. This indicates that in dengue, platelet physiology remains altered even after the febrile phase. CONCLUSION To the best of our knowledge, this is the first study characterizing the differential expression of platelet fatty acids in dengue infection. However, further studies are warranted to expound the underlying cause for thrombocytopenia and platelet dysfunction in dengue.

中文翻译：

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气相色谱-质谱法分析登革热病例中的血小板脂肪酸变化

背景技术登革热病毒在改变血小板功能特性中的作用仍然知之甚少。很少有研究观察到发现脂肪酸的变化会影响血小板的活化和聚集。而且，迄今为止，尚未在登革热中广泛研究血小板脂肪酸。因此，我们旨在研究登革热患者血小板膜的脂肪酸。方法采用气相色谱-质谱法（GC-MS）分析从研究对象中分离出的血小板脂质提取物中的脂肪酸。结果血小板脂质的GC-MS分析鉴定并量化了血小板膜上近23种独特的脂质分子。与对照组相比，我们发现登革热患者的某些脂肪酸发生了显着变化。在登革热病例中，与非严重登革热相比，严重登革热中的不饱和脂肪酸增加。从基线到去热，登革热血小板中未观察到脂肪酸的差异。这表明在登革热中，即使在发热期之后，血小板生理学仍然保持改变。结论据我们所知，这是首次表征登革热感染中血小板脂肪酸差异表达的研究。但是，有必要进行进一步的研究来阐明登革热血小板减少和血小板功能障碍的根本原因。结论据我们所知，这是第一项表征登革热感染中血小板脂肪酸差异表达的研究。但是，有必要进行进一步的研究来阐明登革热血小板减少和血小板功能障碍的根本原因。结论据我们所知，这是第一项表征登革热感染中血小板脂肪酸差异表达的研究。但是，有必要进行进一步的研究来阐明登革热血小板减少和血小板功能障碍的根本原因。