Cancer recurrence and severe side effects of currently being used chemotherapeutic agents reduce their clinical efficacy. Thus, there is a constant need to develop alternative anticancer drugs. Sustainable supply is an important challenge facing marine-based drug discovery. Primmorph, a 3D cell culture system, could provide a sustainable source to produce metabolites for anticancer drugs from marine sponges. In the present work, the anticancer activity of primmorph extracts and mesohyls of Negombata magnifica, Hemimycle arabica, Crella spinulata, and Stylissa carteri sponges was evaluated. Antiproliferative activity was studied in terms of cytotoxicity, colony formation, cell cycle, and apoptosis. Migration was assessed by migration assay and matrix metalloproteinase activity. The expression of proliferation and migration-related genes was analyzed using real time PCR. Migration and proliferation activities of HepG2 cells were inhibited by treatment with primmorph extracts and mesohyls of N. magnifica, H. arabica, and C. spinulata. The mesohyl of S. carteri did not show any anticancer activity although the primmorph extract led to cell cycle arrest. Among the selected sponge species, the primmorph extract of C. spinulata was the most promising anticancer agent regarding antiproliferative and antimigratory activities. In addition, primmorph extracts have the advantage of working under well-defined and controlled conditions, which allows the easy application as a bioreactor.

当前正在使用的化学治疗剂的癌症复发和严重的副作用降低了它们的临床功效。因此，一直需要开发替代的抗癌药。可持续供应是海洋药物发现面临的重要挑战。Primmorph是一种3D细胞培养系统，可以提供可持续的来源，从海洋海绵中生产抗癌药物的代谢产物。在目前的工作中，壮丽的菜豆，半球形的阿拉伯，Crella spinulata和Stylissa carteri的原始形态提取物和中oh的抗癌活性。评估海绵。从细胞毒性，集落形成，细胞周期和细胞凋亡方面研究了抗增殖活性。通过迁移测定和基质金属蛋白酶活性评估迁移。使用实时PCR分析增殖和迁移相关基因的表达。HepG2细胞的迁移和增殖活性通过用primmorph提取物和的mesohyls治疗抑制N. magnifica，H.小粒，和C. spinulata。的mesohyl S. carteri没有表现出任何的抗癌活性虽然primmorph提取物导致细胞周期阻滞。在选定的海绵种类中，刺。的原始提取物就抗增殖和抗迁移活性而言，它是最有前途的抗癌药。此外，primmorph提取物还具有在定义明确和可控的条件下工作的优势，这使其易于用作生物反应器。