One of the most important tools used to evaluate kidney function in the context of chronic kidney disease or other renal function related pathologies is the exploration of glomerular filtration rate (GFR). Iohexol is up to this moment a good candidate molecule for the GFR assessment since it exhibits minimum protein binding rates and minimum extra-renal clearance, being neither secreted nor reabsorbed at the tubular level. This study proposes and evaluates a new LC-MS/MS method for the iohexol determination from capillary blood, prelevated using volumetric absorbative microsampling (VAMS) systems. As an alternative to VAMS, a brand new HemaPEN^® device for micro-prelevation was also tested. A new high throughput sample preparation protocol adapted for iohexol quantification from whole blood VAMS samples was developed. The medium term stability study of iohexol in dried whole blood VAMS samples that was conducted showed a good stability of this molecule for up to 12 days. By collecting only 10 μL of blood, iohexol can be analyzed from dried whole blood VAMS samples for concentration ranges between 1 and 250 μg/mL. Due to the analyte stability in VAMS for up to 12 days, this approach might be successfully applied for GFR assessment for clinical cases allowing minimum invasiveness and even delayed analysis.

在慢性肾脏疾病或其他与肾功能相关的病理情况下用于评估肾功能的最重要工具之一是探索肾小球滤过率（GFR）。到目前为止，碘海醇已成为GFR评估的良好候选分子，因为它显示出最低的蛋白结合率和最低的肾外清除率，在肾小管水平既不分泌也不吸收。这项研究提出并评估了一种新的LC-MS / MS方法，用于从毛细管血中测定碘海醇，使用体积吸收微采样（VAMS）系统进行了改进。作为替代VAMS，一个全新的HemaPEN ^®还测试了微倾斜装置。开发了一种适用于全血VAMS样品中碘海醇定量的新高通量样品制备方案。碘海醇在干燥的全血VAMS样品中进行的中期稳定性研究表明，该分子在长达12天的时间内具有良好的稳定性。通过仅收集10μL血液，可以从干燥的全血VAMS样品中分析碘海醇的浓度范围在1至250μg/ mL之间。由于分析物在VAMS中可保持长达12天的稳定性，因此该方法可以成功地用于临床病例的GFR评估，从而实现最小程度的侵入甚至延迟分析。