The effects of two chlorinated chiral stationary phases, namely, Lux Cellulose-2 and Lux i-Cellulose-5, flow-rate, percentage of co-solvent and chemical structures of the compounds on retention and resolution were studied within this article. In this work a backpressure of 150 bar, a temperature of 40 °C and 10% of methanol as co-solvent were chosen as operating conditions. The optimum flow-rate was 2 mL/min. The percentage of co-solvent was studied between 7.5% and 15%. We have observed that 15% of methanol gave the best results for most of the compounds. For all the derivatives, the Lux Cellulose-2 provided better resolutions going from 1.50 to 3.59 compared with Lux i-Cellulose-5.

本文研究了两种氯化手性固定相，即Lux Cellulose-2和Lux i-Cellulose-5，流速，助溶剂的百分比以及化合物的化学结构对保留和拆分的影响。在这项工作中，选择150 bar的背压，40°C的温度和10％的甲醇作为共溶剂作为操作条件。最佳流速为2 mL / min。研究了助溶剂的百分比在7.5％和15％之间。我们已经观察到，对于大多数化合物，15％的甲醇给出了最佳结果。对于所有衍生物，与Lux i-Cellulose-5相比，Lux Cellulose-2提供的分辨率更高，从1.50到3.59。