Effective methods for managing the oral microbiome are necessary to ensure not only the oral but also the systemic health of a human body. The purpose of this study was to determine the sensitivity of four photosensitizers (PSs) to blue light in six representative oral bacterial species that cause intraoral diseases. The following six strains were investigated: Actinomyces israelii, Enterococcus faecium, Fusobacterium nucleatum, Lactobacillus gasseri, Streptococcus mutans, Veillonella parvula. PS stock solutions (1 mg/ml) were prepared by dissolving curcumin and protoporphyrin-IX in dimethyl sulfoxide, and resazurin and riboflavin in distilled water. The inoculation of 20 ml of a bacterial suspension cultured for 24 hours was mixed with 1,980 ml of each test solution, and then a light source was placed in front of the mixture. The irradiation wavelength was 405 nm and its applied energy was 25.3 J. The independent-samples t-test and one-way analysis of variance within groups were performed to compare the antibacterial effects in the four PSs. The antibacterial susceptibility when using different PSs and visible blue-light irradiation differed between the bacterial strains. Antibacterial photodynamic therapy that includes light exposure and PSs can be used to control the oral bacteria strains related to oral disease.

管理口腔微生物群的有效方法对于确保口腔和人体的全身健康来说是必要的。本研究的目的是确定引起口腔内疾病的六种代表性口腔细菌物种中四种光敏剂（PS）对蓝光的敏感性。研究了以下六种菌株：以色列放线菌、 屎肠球菌、 具核梭杆菌、 加氏乳杆菌、 变形链球菌、 小韦荣球菌。通过将姜黄素和原卟啉-IX 溶解在二甲基亚砜中，将刃天青和核黄素溶解在蒸馏水中来制备 PS 储备溶液 (1 mg/ml)。将20ml培养24小时的细菌悬浮液的接种物与1,980ml各测试溶液混合，然后将光源放置在混合物前面。照射波长为405 nm，照射能量为25.3 J。采用独立样本 t检验和组内单因素方差分析比较4种PS的抗菌效果。使用不同PS和可见蓝光照射时，细菌菌株之间的抗菌敏感性不同。包括光照和 PS 在内的抗菌光动力疗法可用于控制与口腔疾病相关的口腔细菌菌株。