Soybean is an economically important leguminous crop, and pod dehiscence of soybean could cause huge yield loss. In this study, we measured fruit-cracking forces and percentages of dehisced pods for ten soybean accessions, then separated them into two groups as shattering-sensitive (SS) and shattering-resistant (SR) soybeans. Pod transcriptomes from these two groups were analyzed, and 225 differentially expressed genes (DEGs) were identified between SS and SR soybeans. Some of these DEGs have been previously reported to be associated with pod dehiscence in soybean. The expression patterns of selected DEGs were validated by real-time quantitative reverse transcription PCR, which confirmed the expression changes found in RNA-seq analysis. We also de novo identified 246 soybean pod-long intergenic ncRNAs (lincRNAs), 401 intronic lncRNAs, and 23 antisense lncRNAs from these transcriptomes. Furthermore, genes and lincRNAs co-expression network analysis showed that there are distinct expression patterns between SS and SR soybeans in some co-expression modules. In conclusion, we systematically investigated potential genes and molecular pathways as candidates for differences in soybean pod dehiscence and will provide a useful resource for molecular breeding of soybeans.

中文翻译：

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两组大豆之间的破荚特性差异与基因表达的特定变化有关。


大豆是一种重要的经济豆科作物，大豆裂荚可能会造成巨大的产量损失。在这项研究中，我们测量了十个大豆品种的果实破裂力和开裂豆荚的百分比，然后将它们分为两组：破碎敏感（SS）和抗破碎（SR）大豆。对这两组的豆荚转录组进行了分析，并在 SS 和 SR 大豆之间鉴定出了 225 个差异表达基因 (DEG)。其中一些 DEG 此前曾被报道与大豆豆荚开裂有关。通过实时定量逆转录PCR验证了所选DEG的表达模式，证实了RNA-seq分析中发现的表达变化。我们还从这些转录组中从头鉴定了 246 个大豆荚长基因间 ncRNA (lincRNA)、401 个内含子 lncRNA 和 23 个反义 lncRNA。此外，基因和lincRNA共表达网络分析表明，SS和SR大豆在某些共表达模块中存在不同的表达模式。总之，我们系统地研究了大豆荚果开裂差异的潜在基因和分子途径，将为大豆分子育种提供有用的资源。