We investigated the effect of tamoxifen (TAM) treatment on the Notch signaling pathway in mouse ovary. Mice were randomly divided into four groups. Control group A animals were untreated. Control group B animals were treated with the vehicle only. Animals of the 0.5 TAM group received 0.5 mg/day TAM. Animals of the 1.5 TAM group received 1.5 mg/day of TAM. TAM was injected subcutaneously for 5 days. Body weights were measured at the start and end of the experiment. Sections were stained using Crossman's modified trichrome to examine general ovarian structure. Other sections were immunostained to demonstrate Jagged 1, Ki 67 and Notch 2. The TUNEL method was used to detect apoptosis. No significant differences in body weight or ovarian weight were found among the experimental groups. The number of primordial follicles was greater in the treatment groups than in the control groups, while the number of antral follicles and corpora lutea were reduced in the treatment groups. Cell proliferation rates were decreased by TAM treatment and cystic follicles were formed in the ovarian stroma. Notch 2 expression in the granulosa cells was increased following TAM administration, but no change was found in Jagged 1 expression. TAM administration suppressed follicular development and exhibited a negative effect on ovarian morphology. Our findings suggest that the Notch pathway participates in the action of TAM. We suggest that it may be useful to use Notch pathway regulators to adjust the effects of TAM on the ovary.

中文翻译：

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他莫昔芬对小鼠卵巢卵泡Notch信号通路的影响。

我们调查了他莫昔芬（TAM）治疗对小鼠卵巢Notch信号通路的影响。将小鼠随机分为四组。对照组A动物未经治疗。对照组B的动物仅用媒介物治疗。0.5 TAM组的动物接受0.5 mg /天TAM。1.5 TAM组的动物接受1.5 mg /天的TAM。TAM皮下注射5天。在实验开始和结束时测量体重。用Crossman改良的三色染色切片，以检查总体卵巢结构。对其他切片进行了免疫染色，以证明锯齿状1，Ki 67和Notch2。使用TUNEL方法检测凋亡。实验组之间的体重或卵巢重量没有显着差异。在治疗组中，原始卵泡的数量大于对照组，而在治疗组中，窦房卵泡和黄体的数量减少。TAM处理可降低细胞增殖速率，并在卵巢基质中形成囊状卵泡。施用TAM后，颗粒细胞中的Notch 2表达增加，但锯齿状1表达没有变化。TAM给药抑制卵泡发育，并对卵巢形态表现出负面影响。我们的发现表明，Notch途径参与了TAM的作用。我们建议使用Notch通路调节剂来调节TAM对卵巢的作用可能是有用的。而治疗组的肛门卵泡和黄体数量减少了。TAM处理可降低细胞增殖速率，并在卵巢基质中形成囊状卵泡。施用TAM后，颗粒细胞中的Notch 2表达增加，但锯齿状1表达没有变化。TAM给药抑制卵泡发育，并对卵巢形态表现出负面影响。我们的发现表明，Notch途径参与了TAM的作用。我们建议使用Notch通路调节剂来调节TAM对卵巢的作用可能是有用的。而治疗组的肛门卵泡和黄体数量减少了。TAM处理可降低细胞增殖速率，并在卵巢基质中形成囊状卵泡。施用TAM后，颗粒细胞中的Notch 2表达增加，但锯齿状1表达没有变化。TAM给药抑制卵泡发育，并对卵巢形态表现出负面影响。我们的发现表明，Notch途径参与了TAM的作用。我们建议使用Notch通路调节剂来调节TAM对卵巢的作用可能是有用的。但在锯齿状1表达式中未发现任何变化。TAM给药抑制卵泡发育，并对卵巢形态表现出负面影响。我们的发现表明，Notch途径参与了TAM的作用。我们建议使用Notch通路调节剂来调节TAM对卵巢的作用可能是有用的。但在锯齿状1表达式中未发现任何变化。TAM给药抑制卵泡发育，并对卵巢形态表现出负面影响。我们的发现表明，Notch途径参与了TAM的作用。我们建议使用Notch通路调节剂来调节TAM对卵巢的作用可能是有用的。