SummaryWe performed the exposure of bovine oocytes to anethole during in vitro maturation (0 or 300 µg/ml), during in vitro embryo production (0, 30, 300 or 2000 µg/ml), or during both periods to determine the rates of 2−4 cells embryos, blastocysts rates and cells numbers, as well as the production of reactive oxygen species (ROS). Bovine ovaries (n = 240) were collected from a local abattoir after slaughter and cumulus–oocyte complexes (COCs) with homogeneous and non-dark cytoplasm, surrounded by two or more compact layers of cumulus cells, and an intact zona pellucida were selected for in vitro maturatuion (IVM). Mature oocytes were then submitted to in vitro fertilization (IVF) and in vitro embryo production (IVP) in culture medium supplemented or not with different concentrations of anethole, as described above. Although IVM medium supplementation with 300 µg/ml anethole improved the rates of bovine blastocysts formation, we demonstrated that IVP medium supplementation with 30 µg/ml anethole, regardless of IVM medium enrichment, considerably enhanced blastocysts rates. Furthermore, ROS levels were decreased only when anethole was added to the IVP medium without previous IVM medium supplementation.

中文翻译：

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在牛胚胎培养期间而不是在体外成熟培养基中添加茴香脑可提高囊胚率和抗氧化能力

总结我们在牛卵母细胞暴露于茴香脑的过程中进行了体外成熟（0 或 300 µg/ml），在体外胚胎生产（0、30、300 或 2000 µg/ml），或在两个时期内确定 2-4 个细胞胚胎的速率、囊胚率和细胞数量，以及活性氧 (ROS) 的产生。牛卵巢 (n =240）在屠宰后从当地屠宰场收集，卵丘 - 卵母细胞复合物（COC）具有均匀且非深色的细胞质，被两个或更多致密的卵丘细胞层包围，并选择完整的透明带体外成熟（IVM）。然后将成熟的卵母细胞提交给体外受精（IVF）和体外如上所述，在添加或不添加不同浓度茴香脑的培养基中的胚胎生产 (IVP)。尽管添加 300 µg/ml 茴香脑的 IVM 培养基提高了牛胚泡的形成率，但我们证明，无论 IVM 培养基的富集程度如何，添加 30 µg/ml 茴香脑的 IVP 培养基都能显着提高胚泡率。此外，只有在没有先前补充 IVM 培养基的情况下将茴香脑添加到 IVP 培养基中时，ROS 水平才会降低。