After breast and colon cancer, cervical cancer is the third most common cancer of women worldwide. Since human papillomavirus (HPV) infection is known to be the predominant cause of cervical cancer, molecular HPV screening is currently used along with cytological and histological examination methods for precancer diagnosis. Nevertheless, the sensitivity of the current HPV test is less than 80%; thus, many cervical cancer cases are not able to be diagnosed by HPV screening alone, and likewise, patients with cervical cancer are often determined to be HPV-negative by the current screening methods. Therefore, human telomerase reverse transcriptase (hTERT) and Ki67 previously identified as cancer markers were attempted. And cervical exfoliated cells of high-grade squamous intraepithelial lesion (HSIL), the most severe precancerous lesion of cancer, were used in the study. However, it takes a long time to collect enough specimens to conduct statistical analysis. Therefore, in the present study, microscope slides, cervical exfoliated cells on glass slides, were attempted. The results of the analysis demonstrated that hTERT and Ki67 expression levels were useful in distinguishing between cancerous and normal specimens, exhibiting a higher sensitivity and specificity than conventional HPV E6/E7 testing. And the study suggests clinical slide cell samples could be effectively used in the context of retrospective studies to identify novel biomarkers.

中文翻译：

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HPV E6 / E7，hTERT和Ki67 mRNA RT-qPCR分析法，用于用显微镜载玻片检测高度宫颈病变。

继乳腺癌和结肠癌之后，宫颈癌是全球第三大女性癌症。由于已知人类乳头瘤病毒（HPV）感染是子宫颈癌的主要病因，因此分子HPV筛查目前与细胞学和组织学检查方法一起用于癌症前期诊断。尽管如此，当前HPV检测的灵敏度仍低于80％。因此，许多宫颈癌病例不能仅通过HPV筛查来诊断，同样，通过当前的筛查方法，经常将宫颈癌患者确定为HPV阴性。因此，人类端粒酶逆转录酶（hTERT）和Ki67尝试将先前确定为癌症的标志物。研究中使用了最严重的癌前病变高级别鳞状上皮内病变（HSIL）的宫颈脱落细胞。但是，收集足够的样本进行统计分析需要很长时间。因此，在本研究中，尝试了显微镜载玻片，玻璃载玻片上的宫颈脱落细胞。分析结果表明，hTERT和Ki67表达水平可用于区分癌性标本和正常标本，与常规HPV E6 / E7相比，具有更高的灵敏度和特异性测试。这项研究表明，临床滑动细胞样品可以有效地用于回顾性研究中，以鉴定新的生物标志物。