Clubroot is a destructive soil-borne pathogen of Brassicaceae that causes significant recurrent reductions in yield of cruciferous crops. Although there is some resistance in oilseed rape (a crop type of the species Brassica napus), the genetic basis of that resistance is poorly understood. In this study, we used an associative transcriptomics approach to elucidate the genetic basis of resistance to clubroot pathotype ECD 17/31/31 across a genetic diversity panel of 245 accessions of B. napus. A single nucleotide polymorphism (SNP) association analysis was performed with 256,397 SNPs distributed across the genome of B. napus and combined with transcript abundance data of 53,889 coding DNA sequence (CDS) gene models. The SNP association analysis identified two major loci (on chromosomes A2 and A3) controlling resistance and seven minor loci. Within these were a total of 86 SNP markers. Altogether, 392 genes were found in these regions. Another 21 genes were implicated as potentially involved in resistance using gene expression marker (GEM) analysis. After GO enrichment analysis and InterPro functional analysis of the identified genes, 82 candidate genes were identified as having roles in clubroot resistance. These results provide useful information for marker-assisted breeding which could lead to acceleration of pyramiding of multiple clubroot resistance genes in new varieties.

中文翻译：

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通过关联转录组学分析甘蓝型油菜根瘤病抗性变异的遗传结构。

Clubroot是十字花科的一种破坏性土壤传播病原体，会导致十字花科作物的收成大幅减少。尽管油菜（甘蓝型油菜的一种作物类型）具有一定的抗性，但对该抗性的遗传基础了解甚少。在这项研究中，我们使用了一个相关的转录组学方法，阐明了由245个甘蓝型油菜的遗传多样性小组对杆根病原体型ECD 17/31/31产生抗性的遗传基础。使用分布在甘蓝型油菜基因组中的256,397个SNP进行单核苷酸多态性（SNP）关联分析，并与53,889个编码DNA序列（CDS）基因模型的转录本丰度数据相结合。SNP关联分析确定了两个主要基因座（在A2和A3染色体上）控制抗性和七个次要基因座。其中共有86个SNP标记。在这些区域总共发现了392个基因。使用基因表达标记（GEM）分析，推测另外21个基因可能与抗性有关。在对鉴定出的基因进行GO富集分析和InterPro功能分析后，确定了82个候选基因在根瘤菌抗性中起作用。这些结果为标记辅助育种提供了有用的信息，这些信息可能导致新品种中多个根瘤病抗性基因的金字塔化加速。确定了82个候选基因在根瘤菌抗性中起作用。这些结果为标记辅助育种提供了有用的信息，这些信息可能导致新品种中多个根瘤病抗性基因的金字塔化加速。确定了82个候选基因在根瘤菌抗性中起作用。这些结果为标记辅助育种提供了有用的信息，这些信息可能导致新品种中多个根瘤病抗性基因的金字塔化加速。