The aim of this work was to evaluate the ability of broken rice, an underutilized industrial by-product, as a potential functional and health promoting ingredient. With this purpose, the ability to inhibit the angiotensin converting enzyme and renin of a rice protein hydrolyzate (RPH) obtained from a high-protein variety of broken rice (var. Nutriar FCAyF) was analyzed (IC50 = 0.87 and 2.7 mg/mL, respectively). RPH was separated by gel permeation chromatography and in a second purification step by RP-HPLC. The sequence of antihypertensive peptides presented in two RP-HPLC fractions was analyzed. Peptides capable of interacting with the active sites of both enzymes were identified. In this study, we demonstrate that the hydrolysis treatment improves functional and biological properties of rice proteins. Protein preparations obtained from a by-product of rice industry, such as broken rice, are a promising ingredient with potentially good biological properties.

中文翻译：

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碎米作为具有抑制肾素和血管紧张素转换酶 (ACE) 活性的潜在功能成分

这项工作的目的是评估碎米（一种未充分利用的工业副产品）作为潜在的功能性和健康促进成分的能力。为此，分析了从破碎大米 (var. Nutriar FCAyF) 中获得的大米蛋白水解物 (RPH) 抑制血管紧张素转化酶和肾素的能力（IC50 = 0.87 和 2.7 mg/mL，分别）。RPH 通过凝胶渗透色谱分离，并在第二个纯化步骤中通过 RP-HPLC 进行分离。分析了存在于两个 RP-HPLC 级分中的抗高血压肽的序列。鉴定了能够与两种酶的活性位点相互作用的肽。在这项研究中，我们证明水解处理改善了大米蛋白质的功能和生物学特性。