BackgroundChromatin accessibility is crucial for gene expression regulation in specific cells and in multiple biological processes. Assay for Transposase Accessible Chromatin with high-throughput sequencing (ATAC-seq) is an effective way to reveal chromatin accessibility at a genome-wide level. Through ATAC-seq, produced reads from a small number of cells reflect accessible regions that correspond to nucleosome positioning and transcription factor binding sites, due to probing hyperactive Tn5 transposase to DNA sequence.ConclusionIn this review, we summarize both principle and features of ATAC-seq, highlight its applications in basic and clinical research. ATAC-seq has generated comprehensive chromatin accessible maps, and is becoming a powerful tool to understand dynamic gene expression regulation in stem cells, early embryos and tumors.

中文翻译：

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使用 ATAC 测序检测可接近的染色质，从原理到应用

背景染色质可及性对于特定细胞和多种生物过程中的基因表达调控至关重要。使用高通量测序 (ATAC-seq) 检测转座酶可及染色质是在全基因组水平上揭示染色质可及性的有效方法。通过 ATAC-seq，由于将过度活跃的 Tn5 转座酶探查到 DNA 序列，来自少量细胞产生的读数反映了对应于核小体定位和转录因子结合位点的可访问区域。结论在这篇综述中，我们总结了 ATAC- 的原理和特点。 seq，突出其在基础和临床研究中的应用。ATAC-seq 已生成全面的染色质可访问图谱，并且正在成为了解干细胞、早期胚胎和肿瘤中动态基因表达调控的有力工具。