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Mitochondrial genome of Amblyomma javanense: a hard tick parasite of the endangered Malayan pangolin (Manis javanica).
Medical and Veterinary Entomology ( IF 1.6 ) Pub Date : 2019-08-21 , DOI: 10.1111/mve.12403
D-Y Duan 1 , J-M Tang 1 , Z Chen 1 , G-H Liu 1 , T-Y Cheng 1
Affiliation  

Amblyomma javanense is an important ectoparasite of Manis javanica, although the population genetics, molecular biology and systematics of A. javanense remain poorly understood. In the present study, the mitochondrial genome of A. javanense was sequenced using the Illumina HiSeq sequencing platform (Illumina, San Diego, CA, U.S.A.) and compared with the genomes of two closely related species: Amblyomma fimbriatum and Amblyomma americanum. The intraspecies and interspecies relationships of A. javanense and another 21 selected species were investigated by constructing a maximum-likelihood tree and a neighbour-joining tree. The mitochondrial genome of A. javanense was 14 780 bp in length and contained 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and two control regions. The results of the comparisons indicate that there is great similarity among these three species, and both trees indicate that A. javanense is a member of the Amblyomminae. The study of A. javanense of pangolins also indicates the premise and foundation of the relationship between the parasite and other species.

中文翻译:

爪蟾的线粒体基因组:濒临灭绝的马来亚穿山甲(Manis javanica)的硬tick虫。

尽管对爪哇盲the的种群遗传学,分子生物学和系统学知之甚少,但爪哇盲mb仍是爪哇蚊的重要外寄生虫。在本研究中,使用Illumina HiSeq测序平台(Illumina,圣地亚哥,加利福尼亚州,美国)对爪哇拟南芥的线粒体基因组进行了测序,并与两个紧密相关物种的纤毛虫和美洲虫进行了比较。通过构建最大似然树和邻接树,研究了爪哇刺槐和其他21个物种的种内和种间关系。爪哇线粒体的线粒体基因组长度为14 780 bp,包含13个蛋白质编码基因,22个转移RNA基因,两个核糖体RNA基因和两个控制区。比较的结果表明这三个物种之间有很大的相似性,并且两棵树都表明爪哇A. javanense是Amblyomminae的成员。穿山甲的爪哇刺槐的研究也表明了寄生虫与其他物种之间关系的前提和基础。
更新日期:2019-08-21
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