There are seven Vγ gene segments in the TCR γ chain loci of mice. We developed monoclonal antibodies (mAbs) specific to the Vγ6 chain (Heilig & Tonegawa nomenclature). By immunizing Vγ4/6 KO mice with complementarity-determining region peptides in Vγ6 chains, we generated three hybridomas. These hybridomas produced mAbs capable of cell surface staining of Vγ6/Vδ1 gene-transfected T-cell line lacking TCR as well as of Vγ1^- Vγ4^- Vγ5^- Vγ7^- γδ T cells and the CD3^high TCRδ^int γδ T cells in various organs. The location of Vγ6⁺ γδ T cells, which peaked in the newborn thymus, was associated with mTEC. In vivo administration of clone 1C10-1F7 mAb impaired protection against Klebsiella pneumoniae infection but ameliorated psoriasis-like dermatitis induced by imiquimod treatment. These new mAbs are useful to elucidate the development, location, and functions of Vγ6 γδ T cells in mice.

中文翻译：

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新型对小鼠Vγ6链具有特异性的单克隆抗体的开发。

小鼠的TCRγ链基因座中有七个Vγ基因区段。我们开发了对Vγ6链（Heilig和Tonegawa命名法）具有特异性的单克隆抗体（mAb）。通过用Vγ6链中的互补决定区肽免疫Vγ4/ 6 KO小鼠，我们产生了三个杂交瘤。这些杂交瘤产生能缺乏TCR以及Vγ1的Vγ6/Vδ1基因转染的T细胞系的细胞表面染色的单克隆抗体^- Vγ4 ^- Vγ5 ^- Vγ7 ^- γδT细胞和CD3^高TCRδ ^INT γδT细胞在各种器官。Vγ6 ⁺的位置在新生胸腺中达到峰值的γδT细胞与mTEC有关。体内施用克隆1C10-1F7 mAb削弱了对肺炎克雷伯菌感染的保护，但改善了咪喹莫特治疗引起的牛皮癣样皮炎。这些新的单克隆抗体可用于阐明小鼠Vγ6γδT细胞的发育，定位和功能。