Despite significant advances in medicine, mortality due to cardiovascular disease is not yet preventable. We investigated the amounts of elabela (ELA) and apelin, synthesized by cardiomyocytes, and changes of these compounds in cardiac tissue and circulation after administration of iloprost (ILO) and sildenafil (SIL) in rats with induced myocardial ischemia (MI). We also investigated a connection with circulating troponin-I, creatine kinase (CK), creatine kinase-myocardial band (CK-MB) and nitric oxide (NO), and total anti-oxidant (TAS)/total oxidant status (TOS). We established eight study groups of five rats each. Group 1, sham, was given only physiologic serum; group 2, ILO; group 3, SIL; group 4, ILO + SIL; group 5, MI; group 6, MI + ILO; group 7, MI + SIL; group 8, MI + ILO + SIL. Troponin-I, CK, CK-MB and TAS-TOS were investigated using an autoanalyzer. NO, ELA and apelin were analyzed by ELISA. Tissue apelin and ELA expressions and localizations were determined by immunohistochemistry. The MI group compared to the control (sham) group showed that ELA, apelin, troponin-I, CK, CK-MB, NO and TOS levels were elevated significantly. Concentrations of these factors increased in MI, but decreased after ILO and SIL administration. The largest decrease of TOS was identified in the ILO + SIL group. ELA and apelin may be novel indicators of MI and administration of ILO and SIL, individually or together, may be useful for treating MI.

中文翻译：

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伊洛前列素和西地那非对大鼠实验性酶阳性急性冠状动脉综合征中elabela，apelin-13，一氧化氮以及总抗氧化剂和总氧化剂状态的影响。

尽管医学上取得了重大进步，但由于心血管疾病引起的死亡率仍无法预防。我们调查了心肌细胞合成的elabela（ELA）和apelin的量，以及伊洛前列素（ILO）和西地那非（SIL）在诱发心肌缺血（MI）大鼠中的心脏组织和循环中这些化合物的变化。我们还研究了与循环肌钙蛋白-I，肌酸激酶（CK），肌酸激酶-心肌带（CK-MB）和一氧化氮（NO）以及总抗氧化剂（TAS）/总氧化剂状态（TOS）的关系。我们建立了八个研究组，每组五只大鼠。第1组，假的，仅给予生理血清。第2组，国际劳工组织；第3组，SIL；第4组，国际劳工组织+ SIL; 第5组，MI；第6组，MI +国际劳工组织；第7组，MI + SIL；第8组，MI + ILO + SIL。肌钙蛋白-I，CK，使用自动分析仪研究了CK-MB和TAS-TOS。通过ELISA分析NO，ELA和apelin。通过免疫组织化学确定组织apelin和ELA的表达和定位。与对照组（假手术）相比，MI组显示ELA，apelin，肌钙蛋白I，CK，CK-MB，NO和TOS水平显着升高。这些因素的浓度在MI中增加，但在ILO和SIL给药后降低。在ILO + SIL组中，TOS的下降最大。ELA和apelin可能是MI的新指标，ILO和SIL的单独或共同给药对治疗MI可能有用。apelin，肌钙蛋白-I，CK，CK-MB，NO和TOS水平显着升高。这些因子的浓度在MI中增加，但在ILO和SIL给药后降低。在ILO + SIL组中，TOS的下降最大。ELA和apelin可能是MI的新指标，ILO和SIL的单独或共同给药对治疗MI可能有用。apelin，肌钙蛋白-I，CK，CK-MB，NO和TOS水平显着升高。这些因素的浓度在MI中增加，但在ILO和SIL给药后降低。在ILO + SIL组中，TOS的下降最大。ELA和apelin可能是MI的新指标，ILO和SIL的单独或共同给药对治疗MI可能有用。