Calcium-dependent inactivation (CDI) of neuronal voltage-gated Ca²⁺ channels (Ca_V1.2) is important for synaptic plasticity, which is associated with learning and memory. The Ca²⁺-dependent binding of calmodulin (CaM) to Ca_V1.2 is essential for CDI. Here we report NMR assignments for a CaM mutant (D21A/D23A/D25A/E32Q/D57A/D59A/N61A/E68Q, called CaM^EF12) that contains two Ca²⁺ bound at the third and fourth EF-hands (EF3 and EF4) and is bound to the IQ-motif (residues 1644–1665) from Ca_V1.2 (BMRB accession no. 27692).

中文翻译：

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钙调素中间体的化学位移分配与两个 Ca2+ 结合，与电压门控 Ca2+ 通道 (CaV1.2) 的 IQ 基序复合。

神经元电压门控 Ca ²⁺通道 (Ca _V 1.2) 的钙依赖性失活 (CDI) 对于与学习和记忆相关的突触可塑性很重要。钙调蛋白 (CaM) 与 Ca _V 1.2的 Ca ²⁺依赖性结合对于 CDI 至关重要。在这里，我们报告了 CaM 突变体（D21A/D23A/D25A/E32Q/D57A/D59A/N61A/E68Q，称为 CaM ^EF12）的NMR 分配，其中包含在第三和第四个 EF 手（EF3 和 EF4）处结合的两个 Ca ²⁺并与 Ca _V 1.2（BMRB 登录号 27692）的 IQ 基序（残基 1644-1665）结合。