This study describes a perfusable and stretchable culture system for a skin-equivalent. The system is comprised of a flexible culture device equipped with connections that fix vascular channels of the skin-equivalent and functions as an interface for an external pump. Furthermore, a stretching apparatus for the culture device can be fabricated using rapid prototyping technologies, which allows for easy modifications of stretching parameters. When cultured under dynamically stretching and perfusion conditions, the skin-equivalent exhibits improved morphology. The epidermal layer becomes thicker and more differentiated than that cultured without the stretching stimuli or under statically-stretched conditions, and the dermal layer was more densely populated with dermal fibroblasts than that cultured without perfusion due to the nutrient and oxygen supply by perfusion via the vascular channels. Therefore, the system is useful for the improvement and biological studies of skin-equivalents.

中文翻译：

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相当于皮肤的可灌注和可拉伸3D培养系统。

这项研究描述了一种等同于皮肤的可灌洗和可拉伸的培养系统。该系统由一个灵活的培养设备组成，该设备配备了固定相当于皮肤的血管通道的连接，并充当外部泵的接口。此外，可以使用快速成型技术来制造用于培养装置的拉伸设备，这允许容易地改变拉伸参数。当在动态拉伸和灌注条件下培养时，等同于皮肤的皮肤表现出改善的形态。与没有拉伸刺激或在静态拉伸条件下培养的表皮层相比，表皮层变得更厚且更具差异性，真皮层中成纤维细胞的分布比没有灌注时要高，这是由于通过血管通道进行灌注所产生的营养和氧气所致。因此，该系统对于皮肤等效物的改善和生物学研究是有用的。