This study investigated the influence of autophagy on the expression of Collagen type II and light chain 3 (LC-3) in the articular cartilage of osteoarthritis (OA) models. The expression of OA associated biomarkers namely Matrix metalloproteinase (MMP-13), NOD-, LRR- and pyrin domain-containing 3 (NLRP3) induced by destabilizing the medial meniscus operation (DMM) were also investigated. A total of 60 C57BL/6 mice were divided into (1) control; (2) DMM2; (3) DMM8; (4) rapamycin 2 weeks; and (5) rapamycin 8 weeks groups. Saffranin O-Fast green staining, histomorphometry and immunohistochemical methods were used for analysis. In the DMM group, the expression of the OA biomarkers MMP-13, NLRP3 significantly increased, whilst Collagen II and LC-3B levels were significantly lower than other experimental groups. We hypothesized that NLRP3 inhibits autophagy activation and delays disease progression.

中文翻译：

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在小鼠DMM模型中，局部关节内注射雷帕霉素可抑制NLRP3活性并预防骨关节炎。

这项研究调查了自噬对骨关节炎（OA）模型的II型胶原和轻链3（LC-3）表达的影响。还研究了通过破坏内侧半月板操作（DMM）诱导的与OA相关的生物标志物，即基质金属蛋白酶（MMP-13），NOD，LRR和含吡啶结构域的3（NLRP3）的表达。总共60只C57BL / 6小鼠被分为（1）对照。（2）DMM2；（3）DMM8；（4）雷帕霉素2周；（5）雷帕霉素8周组。番红花O-快速绿色染色，组织形态测定法和免疫组化方法用于分析。在DMM组中，OA生物标志物MMP-13，NLRP3的表达显着增加，而II型胶原和LC-3B的水平则明显低于其他实验组。