Context: Osteoarthritis (OA) is a degenerative joint disease with damage to the articular cartilage. Active production of inflammatory cytokine/chemokine and matrix metalloproteinases may be found during the progression of OA. Isorhamnetin had the effects of anti-inflammatory, antioxidant, anti-ischemia, anti-atherosclerotic hepatoprotective and anticancer activities. Objective: Our study was focused on the effects of isorhamnetin treatment in OA. Materials and methods: We used monosodium iodoacetate (MIA)-induced OA rats to evaluate the effects of isorhamnetin related anti-inflammatory process. The rats in all groups were sacrificed on four weeks post-MIA injection. The measurements of knee joint swelling, histological analysis, serum inflammatory biomarkers and western blot were evaluated. Results: We found that isorhamnetin may reduce MIA-induced knee swelling by significantly reduction of articular cartilage damage.in rats. Suppression of pro-inflammatory cytokines production was found after isohamnetin treatment. Isorhamnetin inhibited the production of NO and PGE2, and the expression of iNOS and COX-2. The production of COMP, CTX-II and osteopontin (OPN) were also inhibited in MIA-induced OA rats. Discussion and conclusions: Isorhamnetin may modulate the inflammatory progression of OA in MIA-induced OA rats. The prevention of cartilage damage was found in OA after adequate isorhamnetin treatment. Isorhamnetin may serve as a potential agent for the management of OA.

中文翻译：

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异鼠李素可改善碘乙酸单钠诱导的骨关节炎大鼠的炎症反应和关节软骨损伤。

背景：骨关节炎（OA）是一种退行性关节疾病，会损害关节软骨。在OA进展期间，可能会积极产生炎症细胞因子/趋化因子和基质金属蛋白酶。异鼠李素具有抗炎，抗氧化，抗缺血，抗动脉粥样硬化的肝保护和抗癌作用。目的：我们的研究集中在异鼠李素治疗OA中的作用。材料和方法：我们使用碘乙酸钠（MIA）诱导的OA大鼠评估异鼠李素相关的抗炎过程的作用。在MIA注射后4周将所有组的大鼠处死。评估了膝关节肿胀的测量，组织学分析，血清炎性生物标志物和蛋白质印迹。结果：我们发现异鼠李素可以显着减少大鼠关节软骨的损伤，从而减轻MIA引起的膝关节肿胀。异血红素治疗后，抑制了促炎性细胞因子的产生。异鼠李素抑制NO和PGE2的产生以及iNOS和COX-2的表达。在MIA诱导的OA大鼠中，COMP，CTX-II和骨桥蛋白（OPN）的产生也受到抑制。讨论和结论：异鼠李素可能调节MIA诱导的OA大鼠的OA炎症过程。经过充分异异鼠李素治疗后，OA中可预防软骨损伤。异鼠李素可以作为OA的潜在治疗剂。异鼠李素抑制NO和PGE2的产生以及iNOS和COX-2的表达。在MIA诱导的OA大鼠中，COMP，CTX-II和骨桥蛋白（OPN）的产生也受到抑制。讨论和结论：异鼠李素可能调节MIA诱导的OA大鼠的OA炎症过程。经过充分异异鼠李素治疗后，OA中可预防软骨损伤。异鼠李素可以作为OA的潜在治疗剂。异鼠李素抑制NO和PGE2的产生以及iNOS和COX-2的表达。在MIA诱导的OA大鼠中，COMP，CTX-II和骨桥蛋白（OPN）的产生也受到抑制。讨论和结论：异鼠李素可能调节MIA诱导的OA大鼠的OA炎症过程。经过充分异异鼠李素治疗后，OA中可预防软骨损伤。异鼠李素可以作为OA的潜在治疗剂。