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Functional super-resolution microscopy of the cell.
Current Opinion in Chemical Biology ( IF 6.9 ) Pub Date : 2019-06-15 , DOI: 10.1016/j.cbpa.2019.05.016
Rui Yan 1 , Bowen Wang 1 , Ke Xu 1
Affiliation  

Recent advances in super-resolution fluorescence microscopy have led to ∼10 nm spatial resolution and profound impact on cell biology and beyond. Here we discuss emerging possibilities to encode functional information of intracellular microenvironments, for example, local pH, small-molecule concentrations, chemical polarity (hydrophobicity), and protein activity, into diverse dimensions of the super-resolution signal, hence a class of approaches we collectively refer to as functional super-resolution microscopy. By adding remarkably rich functional information to the already powerful super-resolution arsenal, functional super-resolution microscopy transcends the structural information provided by existing methods, and opens up new ways to unveil fascinating local heterogeneities in live cells with nanometer-scale spatial resolution and ultimate sensitivity down to the single-molecule level.

中文翻译:

细胞的功能超分辨率显微镜。

超分辨率荧光显微镜的最新进展已导致约10 nm的空间分辨率,并对细胞生物学及其他方面产生了深远的影响。在这里,我们讨论了将细胞内微环境的功能信息(例如,局部pH,小分子浓度,化学极性(疏水性)和蛋白质活性)编码为不同分辨率的超分辨率信号的新兴可能性,因此我们提出了一种方法统称为功能超分辨率显微镜。通过向功能强大的超分辨率武器库中添加非常丰富的功能信息,功能超分辨率显微镜超越了现有方法提供的结构信息,
更新日期:2019-11-01
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