Background: Hydrotherapy appears as a valuable therapeutic tool in the management of patients suffering from chronic skin inflammatory diseases. Nevertheless, the underlying immune mechanisms of these beneficial effects remain poorly understood. To better understand the biological effects of thermal spring water on the immune system, we investigated the effects of Avène thermal spring water (ASW) on dendritic cells as key cells participating in the control of the immune response.
Methods: Dendritic cells (DCs) were generated from human monocytes and matured with LPS in ASW-based culture medium or in dexamethasone supplemented culture medium as an anti-inflammatory treatment. The phenotypes and abilities of these DCs to produce cytokines and induce allogeneic T cell response was next assessed.
Results: We showed that ASW modulated the differentiation of monocytes into DCs and impacted the DC maturation upon LPS priming. We observed a reduction of the CD83, CD86, CD1a and HLA-DR molecule expression and a decrease of IL-12 and IL-23 production whereas IL-10 production was increased. LPS-primed DCs generated in presence of ASW exhibited a reduced capacity to induce naive CD4^+, T cell proliferation and IFN-γ and IL-17 production.
Conclusion: Our study showed that ASW is endowed with an immunomodulatory potential. ASW limits the DC stimulatory capacity of Th1 and Th17 cell responses by impairing their maturation, IL-12 and IL-23 production and accessory cell function.



中文翻译：

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温泉水对人树突状细胞炎症反应的影响。

背景：水疗似乎是治疗患有慢性皮肤炎性疾病的患者的一种有价值的治疗工具。然而，这些有益作用的潜在免疫机制仍然知之甚少。为了更好地了解温泉水对免疫系统的生物学影响，我们研究了雅漾温泉水 (ASW) 对树突状细胞的影响，树突状细胞是参与控制免疫反应的关键细胞。
方法：树突状细胞 (DC) 由人类单核细胞产生，并在基于 ASW 的培养基或补充地塞米松的培养基中用 LPS 成熟，作为抗炎治疗。接下来评估这些 DC 产生细胞因子和诱导同种异体 T 细胞反应的表型和能力。
结果：我们发现 ASW 调节单核细胞分化为 DC，并在 LPS 引发时影响 DC 成熟。我们观察到 CD83、CD86、CD1a 和 HLA-DR 分子表达减少，IL-12 和 IL-23 产生减少，而 IL-10 产生增加。在存在 ASW 的情况下产生的 LPS 引发的 DC 表现出诱导幼稚 CD4 ^+、 T 细胞增殖和 IFN-γ 和 IL-17 产生的能力降低。
结论：我们的研究表明，ASW 具有免疫调节潜力。ASW 通过削弱 Th1 和 Th17 细胞的成熟、IL-12 和 IL-23 的产生以及辅助细胞功能来限制 Th1 和 Th17 细胞反应的 DC 刺激能力。