ABSTRACT Melanomas represent the malignant transformation of melanocytes, cells found primarily in the skin to protect epithelium and underlying connective tissues from harmful effects of ultraviolet light. Melanomas vary greatly in morphology and may continue to produce melanin markedly, mildly, or not at all. Performing and evaluating hematoxylin and eosin stains as well as immunohistochemical stains on pigmented melanomas has been a long-standing challenge due to the obscuring pigment. Protocols for removing melanin to reveal cellular morphology have been used successfully for years, but coupling these protocols with stains for immunohistochemistry represents an added challenge. In this study, the investigators evaluated results of various melanin bleaching protocols on tissue morphology, completeness of melanin removal, and immunohistochemistry staining quality. It was found that 1% formamide in 3% H2O2 under bright light without heating outperformed other tested protocols.

中文翻译：

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在色素性黑色素瘤中使用苏木精和伊红染色和免疫组织化学增殖标志物 MCM3 的漂白方案的比较

摘要黑色素瘤代表黑色素细胞的恶性转化，黑色素细胞主要存在于皮肤中，以保护上皮和下层结缔组织免受紫外线的有害影响。黑色素瘤的形态差异很大，可能会继续显着、轻微或根本不产生黑色素。由于色素模糊，在色素性黑色素瘤上执行和评估苏木精和伊红染色以及免疫组织化学染色一直是一项长期挑战。去除黑色素以揭示细胞形态的方案已成功使用多年，但将这些方案与染色剂结合用于免疫组织化学是一个额外的挑战。在这项研究中，研究人员评估了各种黑色素漂白方案对组织形态、黑色素去除完整性、和免疫组化染色质量。发现在强光下不加热的 3% H2O2 中的 1% 甲酰胺优于其他测试方案。