ABSTRACT The study investigated the effect of extremely low-frequency electromagnetic fields (ELF-EMFs) exposure at different magnetic flux densities on genes expression of transcription factor Maf (c-Maf), signal transducer and activator of transcription 6 (STAT6), and retinoid-related orphan receptor alpha (RORα) in the spleen and thymus of rats. Eighty adult male rats were separated into four ELF-EMFs exposed and were exposed to magnetic flux densities of 1, 100, 500, and 2000 µT at a frequency of 50 Hz for 2 h daily for up to 60 d. All rats were intraperitoneally immunized on d 31, 44, and 58 of exposure. The experimental results showed that the expression levels of c-Maf, STAT6, and RORα in the thymus were not significantly changed at different magnetic flux densities. The expression levels of RORα and c-Maf were significantly downregulated at the densities of 1 and 100 µT, while the expression of STAT6 was only significantly decreased at the density of 100 µT. In conclusion, low magnetic flux densities of ELF-EMFs may reduce the expression levels of c-Maf, STAT6, and RORα genes in the spleen.

中文翻译：

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极低频电磁场对大鼠脾、胸腺c-Maf、STAT6、RORα表达的影响

摘要 本研究调查了不同磁通密度下极低频电磁场 (ELF-EMF) 暴露对转录因子 Maf (c-Maf)、信号转导和转录激活剂 6 (STAT6) 和类视黄醇基因表达的影响大鼠脾脏和胸腺中的 -相关孤儿受体α（RORα）。80 只成年雄性大鼠被分成 4 个暴露的 ELF-EMF，并暴露于 1、100、500 和 2000 µT 的磁通量密度，频率为 50 Hz，每天 2 小时，持续时间长达 60 天。在暴露的第 31、44 和 58 天对所有大鼠进行腹膜内免疫。实验结果表明，不同磁通密度下胸腺中c-Maf、STAT6和RORα的表达水平无明显变化。RORα 和 c-Maf 的表达水平在 1 和 100 µT 的密度下显着下调，而 STAT6 的表达仅在 100 µT 的密度下显着降低。总之，ELF-EMF 的低磁通密度可能会降低脾脏中 c-Maf、STAT6 和 RORα 基因的表达水平。