ABSTRACT This paper presents a method to inhibit B16F10 cancer cells using extremely low-frequency electromagnetic fields (ELF-EMFs) and to evaluate cell viability using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. The study examined the effect of a natural EMF resonance frequency (7.83 Hz) and a power line frequency (60 Hz) on B16F10 cancer cells for 24 and 48 h. The B16F10 cancer cells were also exposed to sweep frequencies in several sweep intervals to quantitatively analyze the viability of cancer cells. The results yielded a 17% inhibition rate under 7.83 Hz compared with that of the control group. Moreover, sweep frequencies in narrow intervals (7.83 ± 0.1 Hz for the step 0.05 Hz) caused an inhibition rate of 26.4%, and inhibitory effects decreased as frequency sweep intervals increased. These results indicate that a Schumann resonance frequency of 7.83 Hz can inhibit the growth of cancer cells and that using a specific frequency type can lead to more effective growth inhibition.

中文翻译：

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极低频电磁场对B16F10癌细胞的影响

摘要 本文介绍了一种使用极低频电磁场 (ELF-EMF) 抑制 B16F10 癌细胞并使用 MTT (3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑）测定。该研究检查了自然 EMF 共振频率 (7.83 Hz) 和电源线频率 (60 Hz) 对 B16F10 癌细胞的影响 24 和 48 小时。B16F10 癌细胞还以几个扫描间隔暴露于扫描频率，以定量分析癌细胞的活力。结果与对照组相比，在7.83Hz下产生了17%的抑制率。此外，窄间隔扫描频率（7.83 ± 0.1 Hz for step 0.05 Hz）的抑制率为 26.4%，并且随着频率扫描间隔的增加，抑制效果降低。