The aim of this study was to identify candidate regions associated with sexual precocity in Bos indicus. Nellore and Brahman were set as validation and discovery populations, respectively. SNP selected in Brahman to validate in Nellore were from gene regions affecting reproductive traits (G1) and significant SNP (p ≤ 10-3 ) from a meta-analysis (G2). In the validation population, early pregnancy (EP) and scrotal circumference (SC) were evaluated. To perform GWAS in validation population, we used regression and Bayes C. SNP with p ≤ 10-3 in regression and Bayes factor ≥3 in Bayes C were deemed significant. Significant SNP (for EP or SC) or SNP in their ±250 Kb vicinity region, which were in at least one discovery set (G1 or G2), were considered validated. SNP identified in both G1 and G2 were considered candidate. For EP, 145 SNP were validated in G1 and 41 in G2, and for SC, these numbers were 14 and 2. For EP, 21 candidate SNP were detected (G1 and G2). For SC, no candidate SNP were identified. Validated SNP and their vicinity region were located close to quantitative trait loci or genes related to reproductive traits and were enriched in gene ontology terms related to reproductive success. These are therefore strong candidate regions for sexual precocity in Nellore and Brahman.

中文翻译：

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跨品种验证研究证实并确定了与婆罗门牛和内洛尔牛性早熟相关的新位点

本研究的目的是确定与 Bos indicus 性早熟相关的候选区域。Nellore 和 Brahman 分别被设置为验证和发现群体。在 Brahman 中选择以在 Nellore 中验证的 SNP 来自影响生殖性状 (G1) 的基因区域和来自荟萃分析 (G2) 的显着 SNP (p ≤ 10-3)。在验证人群中，评估了早孕 (EP) 和阴囊围 (SC)。为了在验证群体中执行 GWAS，我们使用了回归和贝叶斯 C。回归中 p ≤ 10-3 的 SNP 和贝叶斯 C 中的贝叶斯因子 ≥ 3 被认为是显着的。显着的 SNP（对于 EP 或 SC）或在其 ±250 Kb 附近区域中的 SNP，至少在一个发现集（G1 或 G2）中，被认为是经过验证的。在 G1 和 G2 中鉴定的 SNP 被认为是候选者。对于 EP，在 G1 中验证了 145 个 SNP，在 G2 中验证了 41 个，对于 SC，这些数字是 14 和 2。对于 EP，检测到 21 个候选 SNP（G1 和 G2）。对于 SC，未鉴定出候选 SNP。经验证的 SNP 及其附近区域位于数量性状基因座或与生殖性状相关的基因附近，并富含与生殖成功相关的基因本体术语。因此，这些是 Nellore 和 Brahman 性早熟的有力候选区域。