We investigated the mechanisms by which propiverine hydrochloride influenced bladder activity in rats with pelvic venous congestion (PC) and urinary frequency. To create PC rats, female rats were anesthetized with isoflurane and the bilateral common iliac veins and bilateral uterine veins were ligated. At 4 weeks after ligation, we assessed voiding behaviour, locomotor activity, and urinary 8-hydroxydeoxyguanosine (8-OHdG) and nitric oxide metabolites (NOx). We also performed cystometry and measured mRNAs for nitric oxide synthase (NOS) and several receptors in the bladder wall. PC rats showed a decrease in locomotor activity and an increased frequency of urination. There was a decrease in endothelial NOS (eNOS), M3, and TRPV1 mRNA expression in the bladder wall, as well as an increase in inducible NOS (iNOS) mRNA. Administration of propiverine to PC rats increased locomotor activity to the level in sham rats, improved bladder function, decreased urinary 8-OHdG excretion, and increased urinary NOx excretion. In addition, propiverine increased neuronal NOS (nNOS) mRNA expression, and decreased expression of iNOS, M3 and TRPV1 mRNA in the bladder wall. Therefore, propiverine not only improved bladder dysfunction through its previously reported actions (anti-muscarinic effect, Ca antagonist effect, and inhibition of noradrenaline re-uptake), but also by reducing inflammation.

中文翻译：

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异丙肾上腺素对盆腔静脉充血和尿频的大鼠膀胱活动的影响的潜在机制。

我们调查了盐酸普萘匹林影响盆腔静脉充血（PC）和尿频的大鼠膀胱活动的机制。为了制造PC大鼠，用异氟烷麻醉雌性大鼠，并结扎双侧common总静脉和双侧子宫静脉。结扎后第4周，我们评估了排尿行为，运动能力以及尿中的8-羟基脱氧鸟苷（8-OHdG）和一氧化氮代谢产物（NOx）。我们还进行了膀胱测压，并测量了一氧化氮合酶（NOS）和膀胱壁中几个受体的mRNA。PC大鼠表现出自发活动的减少和排尿频率的增加。膀胱壁内皮NOS（eNOS），M3和TRPV1 mRNA表达减少，诱导型NOS（iNOS）mRNA增加。给予PC大鼠丙肝素可增加运动能力，达到假大鼠的水平，改善膀胱功能，减少尿中8-OHdG的排泄，并增加尿中NOx的排泄。此外，丙肝素增加了膀胱壁神经元NOS（nNOS）mRNA的表达，并降低了iNOS，M3和TRPV1 mRNA的表达。因此，普罗维林不仅可以通过先前报道的作用（抗毒蕈碱作用，Ca拮抗剂作用和抑制去甲肾上腺素的再摄取）改善膀胱功能障碍，而且还可以减少炎症。膀胱壁中的M3和TRPV1 mRNA。因此，普萘匹林不仅可以通过先前报道的作用（抗毒蕈碱作用，Ca拮抗剂作用和抑制去甲肾上腺素的再摄取）改善膀胱功能障碍，而且还可以减少炎症。膀胱壁中的M3和TRPV1 mRNA。因此，普萘匹林不仅可以通过先前报道的作用（抗毒蕈碱作用，Ca拮抗剂作用和抑制去甲肾上腺素的再摄取）改善膀胱功能障碍，而且还可以减少炎症。