In this study, we attempted to localize the immunoreactivities of podoplanin/E11/gp38 and CD44, a counterpart possessing a high affinity to podoplanin/E11/gp38, as well as endomucin-immunoreactive blood vessels in the regions of odontoblast layers and the underlying sub-odontoblastic layers in murine tooth germs. Endomucin-reactive small blood vessels were scattered throughout the dental papillae of the tooth germs at postnatal day 1 but came to be localized close to the odontoblast/sub-odontoblastic layers until day 3. After postnatal day 5, small blood vessels were seen in odontoblast cell layers, while blood vessels with relatively larger diameters were seen forming in sub-odontoblastic layers. Immunoreactivities of podoplanin/E11/gp38 and CD44 were not detectable in the cells of dental papillae facing the inner enamel epithelium at postnatal day 1. However, at around postnatal days 3-5, podoplanin/E11/gp38 was localized in the odontoblast layer but not in the sub-odontoblastic layer, whereas CD44 was observed in the sub-odontoblastic layer but not in the odontoblast layer. The exclusive immunolocalization of podoplanin/E11/gp38 and CD44 in the odontoblast layers and sub-odontoblastic layers was seen after postnatal day 3 of the tooth germs, when the mesenchymal cells of dental papillae have already differentiated into mature odontoblasts at the cusp tip. Taken together, it seems likely that endomucin-reactive small blood vessels extended to the podoplanin/E11/gp38-positive odontoblast layers, whereas endomucin-reactive large blood vessels were already present in CD44-immmunopositive sub-odontoblastic layer, indicating the cellular regulation on the vascularization of endomucin-reactive endothelial cells during odontogenesis of the tooth germs.

中文翻译：

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Podoplanin / E11 / gp38，CD44和内粘蛋白在鼠牙胚成牙本质细胞层中的免疫定位。

在这项研究中，我们试图定位podoplanin / E11 / gp38和CD44的免疫反应性，其对应物对podoplanin / E11 / gp38具有高亲和力，并且在成牙本质细胞层和下层亚区的内粘蛋白免疫反应性血管鼠牙胚中的成牙本质细胞层。在出生后第1天，对内源性粘蛋白有反应的小血管散布在整个牙齿细菌的牙乳头中，但直到第3天才定位于成牙本质细胞/成牙本质亚层附近。在出生后第5天，成牙本质细胞中可见小血管。细胞层，而在亚齿质层中则发现有直径较大的血管。在出生后第1天，面对内釉上皮的牙乳头细胞中未检测到podoplanin / E11 / gp38和CD44的免疫反应性。但是，在出生后第3-5天，podoplanin / E11 / gp38定位在成牙本质层中，但在牙本质成骨细胞层中没有观察到CD44，而在牙本质成骨细胞层中观察到了CD44，而在成牙本质细胞层中没有观察到CD44。出生后第3天，当牙乳头的间充质细胞已经分化成尖尖的成年成牙本质细胞时，podoplanin / E11 / gp38和CD44在成牙本质细胞层和成牙本质细胞层中的唯一免疫定位被发现。综上所述，内粘蛋白反应性小血管似乎延伸到了podoplanin / E11 / gp38阳性成牙本质细胞层，