We studied the level of spontaneous telomere dysfunction in Rattus norvegicus (Berkenhout, 1769) (Rodentia, Muridae) embryonic fibroblasts (rEFs) and in cultured in vitro rat pluripotent stem cells (rPSCs), embryonic stem cells (rESCs) and induced pluripotent stem cells (riPSCs), on early passages and after prolonged cultivation. Among studied cell lines, rESCs showed the lowest level of telomere dysfunction, while the riPSCs demonstrated an elevated level on early passages of cultivation. In cultivation, the frequency of dysfunctional telomeres has increased in all studied cell lines; this is particularly true for dysfunctional telomeres occurring in G1 stage in riPSCs. The obtained data are mainly discussed in the connection with the specific structure of the telomere regions and their influence on the differential DNA damage response in them.

中文翻译：

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胚胎成纤维细胞和体外培养的褐家鼠（啮齿动物，鼠科）多能干细胞中端粒功能障碍。


我们研究了褐家鼠 (Berkenhout, 1769)（啮齿目、鼠科）胚胎成纤维细胞 (rEF) 以及体外培养的大鼠多能干细胞 (rPSC)、胚胎干细胞 (rESC) 和诱导多能干细胞中自发端粒功能障碍的水平（riPSC），在早期传代和长期培养后。在所研究的细胞系中，rESC 显示出最低水平的端粒功能障碍，而 riPSC 在培养的早期传代中显示出较高水平的端粒功能障碍。在培养过程中，所有研究的细胞系中端粒功能失调的频率均有所增加；对于 riPSC 中 G1 阶段发生的功能失调的端粒来说尤其如此。所获得的数据主要讨论端粒区域的具体结构及其对端粒区域差异DNA损伤反应的影响。