Surface modification by non-thermal atmospheric plasma (NTAP) treatment can produce significantly higher carboxylic groups on the nanofibers (NF) surface, which potentially can increase biomineralization of NF via promoting glutamic acid (GLU) templated peptide conjugation. Herein, electrospun poly(lactide-co-glycolide) (PLGA) scaffolds were treated with NTAP and conjugated with GLU peptide followed by incubation in simulated body fluids for mineralization. The effect of NTAP treatment and GLU peptide conjugation on mineralization, surface wettability and roughness were investigated. The results showed that NTAP treatment significantly increased GLU peptide conjugation which consequently enhanced mineralization and mechanical properties of NTAP treated and peptide conjugated NF (GLU-pNF) compared to neat PLGA NF, NTAP treated NF (pNF) and GLU peptide conjugated NF (GLU-NF). The effect of surface modification on human bone marrow derived mesenchymal stem cells adhesion, proliferation and morphology was evaluated by cell proliferation assay and fluorescent microscopy. Results demonstrated that cellular adhesion and proliferation were significantly higher on GLU-pNF compared to NF, pNF and GLU-NF. In summary, NTAP treatment could be a promising modification technique to induce biomimetic peptide conjugation and biomineralization for bone tissue engineering applications.

中文翻译：

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通过非热大气等离子体辅助谷氨酸模板肽缀合加速纳米纤维的矿化。

通过非热大气等离子体 (NTAP) 处理进行表面改性可以在纳米纤维 (NF) 表面产生显着更高的羧基，这可能通过促进谷氨酸 (GLU) 模板肽缀合来增加 NF 的生物矿化。在此，电纺聚丙交酯乙交酯 (PLGA) 支架经过 NTAP 处理并与 GLU 肽缀合，然后在模拟体液中孵育进行矿化。研究了 NTAP 处理和 GLU 肽缀合对矿化、表面润湿性和粗糙度的影响。结果表明，NTAP 处理显着增加了 GLU 肽缀合，从而与纯 PLGA NF、NTAP 处理的 NF (pNF) 和 GLU 肽缀合 NF (GLU- NF）。通过细胞增殖实验和荧光显微镜评估表面修饰对人骨髓间充质干细胞粘附、增殖和形态的影响。结果表明，与 NF、pNF 和 GLU-NF 相比，GLU-pNF 上的细胞粘附和增殖显着更高。总之，NTAP 处理可能是一种有前景的修饰技术，可诱导仿生肽缀合和生物矿化，用于骨组织工程应用。