Background

Rice (Oryza sativa L.) is one of the major staple food crops consumed globally. However, rice production is severely affected by high salinity levels, particularly at the seedling stage. A good solution would be the development of an efficient screening methodology to identify genotypes possessing genes for salt tolerance.

Result

A new salinity tolerance screening technique using rice seedlings in pot-culture was tested. This method controls soil heterogeneity by using pure sand as a growth medium and minimizes unexpected extreme weather conditions with a movable shelter. Seventy-four rice genotypes were screened at three salinity treatments including high salt stress (electrical conductivity (EC) 12 dSm^− 1), moderate salt stress (EC 6 dSm^− 1), and control (no salt stress), imposed 1 week after emergence. Several shoot and root morpho-physiological traits were measured at 37 days after sowing. A wide range of variability was observed among genotypes for measured traits with root traits being identified as the best descriptors for tolerance to salt stress conditions. Salt stress response indices (SSRI) were used to classify the 74 rice genotypes; 7 genotypes (9.46%) were identified as salt sensitive, 27 (36.48%) each as low and moderately salt tolerant, and 13 (17.57%) as highly salt tolerant. Genotypes FED 473 and IR85427 were identified as the most salt tolerant and salt sensitive, respectively. These results were further confirmed by principal component analysis (PCA) for accuracy and reliability.

Conclusion

Although tolerant genotypes still need to be confirmed in field studies and tolerance mechanisms identified at the molecular level, information gained from this study could help rice breeders and other scientists to accelerate breeding by selecting appropriate donor parents, progenies and potential genotypes at early growth stages necessary for salinity tolerance research.

中文翻译：

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使用盆栽法评估水稻的盐度可在苗期进行系统的耐性评估。

背景

稻米（Oryza sativa L。）是全球消费的主要主粮作物之一。然而，高盐度严重影响水稻生产，特别是在苗期。一个好的解决方案是开发一种有效的筛选方法，以鉴定具有耐盐基因的基因型。

结果

测试了一种在盆栽中使用水稻幼苗的新的耐盐性筛选技术。该方法通过使用纯砂作为生长介质来控制土壤异质性，并通过可移动的遮挡物将意外的极端天气条件降至最低。在三种盐度处理下筛选了74种水稻基因型，包括高盐胁迫（电导率（EC）12 dSm ^{− 1}），中度盐胁迫（EC 6 dSm ^{− 1）。}），并在出苗后1周内进行控制（无盐胁迫）。播种后第37天测量了几种芽和根的形态生理特性。观察到的性状在基因型之间观察到广泛的变异性，根性状被认为是耐盐胁迫条件的最佳描述。盐胁迫反应指数（SSRI）被用来分类74个水稻基因型。鉴定出7个基因型（9.46％）为盐敏感型，其中27个（36.48％）为低和中度耐盐性，13个（17.57％）为高度耐盐性。基因型FED 473和IR85427分别被确定为最耐盐和最耐盐的。通过主成分分析（PCA）进一步证实了这些结果的准确性和可靠性。

结论

尽管仍需要在田间研究中确认耐性基因型并在分子水平上确定耐性机制，但从这项研究中获得的信息可以通过选择合适的供体亲本，后代和潜在的基因型，帮助水稻育种者和其他科学家加速育种。用于耐盐性研究。