It is unclear if naïve T cells require dendritic cell ICAMs to proliferate inside lymph nodes. To check if and when CD4 lymphocytes use ICAMs on migratory DCs, wild-type and ICAM-1 and 2 double knock out bone marrow-derived DCs pulsed with saturating levels of an OT-II transgene-specific ovalbumin-derived peptide were co-transferred into skin-draining lymph nodes. Intravital imaging of OT-II lymphocytes entering these lymph nodes revealed that ICAM-1 and -2 deficient migratory DCs formed fewer stable conjugates with OT-II lymphocytes but promoted normal T cell proliferation. DC ICAMs were also not required for unstable TCR-dependent lymphocyte arrests on antigen presenting migratory DCs. Thus, rare antigen-stimulated ICAM-stabilized T-DC conjugates are dispensable for CD4 lymphocyte proliferation inside lymph nodes.

中文翻译：

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幼稚 CD4 T 细胞与迁移树突状细胞的稳定接触依赖于 ICAM-1，但对于体内增殖来说是可有可无的。


目前尚不清楚幼稚 T 细胞是否需要树突状细胞 ICAM 在淋巴结内增殖。为了检查 CD4 淋巴细胞是否以及何时在迁移 DC 上使用 ICAM，将野生型以及 ICAM-1 和 2 双敲除的骨髓衍生 DC 与饱和水平的 OT-II 转基因特异性卵清蛋白衍生肽进行共转移进入皮肤引流淋巴结。进入这些淋巴结的 OT-II 淋巴细胞的活体成像显示，ICAM-1 和 -2 缺陷的迁移 DC 与 OT-II 淋巴细胞形成的稳定结合物较少，但促进了正常 T 细胞增殖。 DC ICAM 也不需要抗原呈递迁移 DC 上不稳定的 TCR 依赖性淋巴细胞停滞。因此，罕见的抗原刺激的 ICAM 稳定的 T-DC 缀合物对于淋巴结内 CD4 淋巴细胞的增殖来说是可有可无的。