Actin has been shown to be essential for clathrin‐mediated endocytosis in yeast. However, actin polymerization alone is likely insufficient to produce enough force to deform the membrane against the huge turgor pressure of yeast cells. In this paper, we used Brownian dynamics simulations to demonstrate that crosslinking of a meshwork of nonpolymerizing actin filaments is able to produce compressive forces. We show that the force can be up to several thousand pico‐Newtons if the crosslinker has a high stiffness. The force decays over time as a result of crosslinker turnover, and is a result of converting chemical binding energy into elastic energy.

中文翻译：

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交联肌动蛋白网络产生压缩力。


肌动蛋白已被证明对于酵母中网格蛋白介导的内吞作用至关重要。然而，单独的肌动蛋白聚合可能不足以产生足够的力来使膜变形以抵抗酵母细胞的巨大膨胀压力。在本文中，我们使用布朗动力学模拟来证明非聚合肌动蛋白丝网状结构的交联能够产生压缩力。我们表明，如果交联剂具有高刚度，则力可高达数千皮牛顿。由于交联剂周转，力随着时间的推移而衰减，并且是将化学结合能转化为弹性能的结果。